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The KIT houses the largest experimental fish facility in Europe with a capacity of more than 300,000 fish and more than 10 research groups working with zebrafish at KIT. It was thus logical to enlarge the facility further to serve as the European Zebrafish Resource Center (EZRC). In July 2012, we officially opened the EZRC. Zebrafish stocks are maintained mostly as frozen sperm. Frequently requested lines are also kept alive as well as a selection of wildtype strains. We have established efficient pipelines for sperm freezing, thawing and developed a database to organise the complex information. We hold several thousand mutations in protein coding genes generated by TILLING in the Stemple lab of the Sanger Centre, Hinxton, UK and lines generated by ENU mutagenesis by the Nüsslein-Volhard lab in addition to transgenic lines and mutants generated by KIT groups or brought in through collaborations. We also accept submissions on an individual basis and ship fish upon request to PIs in Europe and elsewhere.
The EZRC does not only maintain and distribute a large number of existing mutant and transgenic zebrafish lines but also provides screening services and technologies such as imaging and high-throughput sequencing. In close interaction with physicists, engineers and IT specialists, we expanded the medium/high throughput screening technologies that have been recently established at KIT. Key areas include automation of embryo handling and automated image acquisition and processing. Our platform also involves the development of novel microscopy techniques (e.g. SPIM, DSLM, robotic macroscope) to permit high-resolution, real-time imaging in 4D. By association with the ComPlat platform, we can support also chemical screens and offer libraries with up to 20,000 compounds in total for external users. As another service to the community the EZRC provides plasmids (cDNAs, transgenes, Talen, Crispr/cas9) maintained by the Helmholtz repository of Bioparts (HERBI) to the scientific community.
Company Details
- Founded
- -
- Address
- Hermann-Von-Helmholtz-Platz, Eggenstein-Leopoldshafen,baden-Württemberg 76344,germany
- Industry
- Biotechnology
- Website
- http://www.ezrc.kit.edu/
- Keywords
- Zebrafish husbandry, Cryopreservation, in vitro fertilisation, Genotyping, Transgenesis, Mutagenesis.
- HQ
- Eggenstein-Leopoldshafen, Baden-Württemberg
European Zebrafish Resource Center - Ezrc Questions
European Zebrafish Resource Center - EZRC's website is http://www.ezrc.kit.edu/
European Zebrafish Resource Center - EZRC's LinkedIn profile is https://de.linkedin.com/company/ezrc
European Zebrafish Resource Center - EZRC's industry is
Biotechnology
European Zebrafish Resource Center - EZRC's top competitors are
Zebrafish,
Zebrafish Community Idea Bio-Medical,
Zebrafish Researchers Group,
Zebrafish International Resource Center (Zirc),
French Zebrafish Meeting,
Aroora Centre For Preclinical Research,
Zebrafish,
Zebrafish Disease Models Society,
Zebrafish Rock!,
European Zebrafish Society.
European Zebrafish Resource Center - EZRC's categories are Biotechnology
European Zebrafish Resource Center - EZRC's founding year is 2012
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