Research and development of new approaches to produce dairy products in an alternative and sustainable way. Research of effective cell culture methods, aimed at the production of dairy proteins, through the implementation of advanced cell culture media and techniques, appropriate for the purpose of the projects.

Ph.D. in Cell Biology focused on human diseases. I adopted and optimized cutting-edge technologies, including: • light sheet fluorescence microscopy (LSFM) clearing techniques (CUBIC, Scale), tissues antibodies panels staining. • bulk / single-cell and single-nuclei RNA sequencing • droplet and split-pool-based • CUT&RUN and library preparation• in-vitro metabolic assay (Seahorse Analysis) • flow cytometry • cell sorting• metagenomicsCombined with in vitro cell assays in a 2D fashion adopting: • nephron microdissection culture • murine primary cell culture • immortalized cell lines culture • transfection • migration assaysand standardized basic technics: • high-resolution confocal microscopy with fixed tissue or live cell• multi-immunofluorescence staining panels • immunohistochemistry and histochemistry• immunoprecipitation assay• qPCR and PCR • ELISA• protein analysis via WBI have worked with both in-vivo and ex-vivo methods:• ischemia-reperfusion injury, nephrectomy, and diabetic mouse models• drugs screening • microsurgery • heart, abdominal aorta, and kidney perfusion• ex vivo cilia motility assay in mouse tracheal tissue• tissues preparation • embedding, cryopreservationSupervised students and collaborated with groups in different fields from cardiology, immunology to neurology.

Nine months in England, sponsored by the Erasmus Project, allowed me to conduct my thesis project on lost skeletal muscle and autophagy. I improved my technical skills in the biological laboratory, including biochemical and molecular methods. During that year I published an article: https://pubmed.ncbi.nlm.nih.gov/29899362/ and I expanded my networking by making national and international connections.