Department of Physiology and Pharmacology /Hypertension and Vascular Research Center

● Planned and organized research project to investigate the use of feed additives to reduce enteric methane emissions.● Performed extensive literature review to justify the relevance of reducing enteric methane production in dairy cows.● Developed and validated analytical methods to measure in vitro methane gas production from microbial fermentation.● Created standard laboratory protocols to prepare chemicals and solvents for all experiments.● Investigated, analyzed, and recorded experimental data from in vitro culture studies.● Performed field and laboratory sample collection.● Operated and maintained Picarro G2301 CH4/CO2/ H2O analyzer (Cavity Ring-Down (laser) Spectroscopy) to quantify gas concentrations.● Supervised, mentored, and assisted graduate and undergraduate research assistants on laboratory techniques, safety training, data collection, and data interpretation.● Taught ANSC 611 Animal Nutrition upon request of advisor.● Validated microbial DNA extraction technique to identify methanogens in the rumen.● Performed agarose gel electrophoresis and real-time polymerase chain reactions (PCR) using SYBR green to measure genomic DNA amplification using Bio-Rad Thermocycler in response to the addition of feed additives.● Performed statistical analysis using GraphPad Prism. ● Co-mentored 1graduate and 3 undergraduate students.●Analyze simple mathematical algorithms/models to predict CH4 and CO2 gas concentrations.

● Course: Organic Chemistry I● Class size: 30● Planned and organized course of study for Organic Chemistry I Lecture Course.● Served office hours for Organic Chemistry I Lecture Course. ● Taught Organic Chemistry I Laboratory Course.

● Planned and organized the overall research project to investigate the impacts of land use on water quality.● Addressed scientific concerns, collected and interpreted data on stream water quality of the Jordan Lake and Falls Lake watersheds.● Used Geographical Information Systems to investigate land use classifications on fecal coliform, trace element concentrations in stream water● Collaborated and established professional relationships with the Stormwater Services Division in Durham County, NC to collect and analyze stream water quality data.● Documented land uses that impact stream water quality in Durham County, NC.