Antonio Câmara Email & Phone Number

Nancy, Grand Est, France

Région De Campinas, Brésil

- Construction of a collection of over 1300 yeast strains from more than 580 Brazilian cheeses.- Development of a molecular screening protocol (RAPD, PCR).- Development of a screening protocol for strains under acid, thermal, and water stress.- Development of a heat shock protocol and a strain storage protocol under controlled humidity and temperature conditions.- Data analysis: R, Bionumerics, Mega 11, predictive microbiology, modeling.- Management of the Molecular Biology laboratory: ensuring compliance with safety standards, personnel training, waste management, ordering consumables.- Management of the four-year budget for the project funded by FAPESP, São Paulo.- Supervision of two German interns.- Two book chapters accepted.- One paper published.

Gif-Sur-Yvette, Île-De-France, France

- Comparative genomics of yeast under stress conditions.- Whole genome sequencing of 20 selected yeasts.- Preparation of DNA samples for sequencing/genomics, purification, quality control, electrophoresis.- Microorganism typing, PCR.- Data analysis: CodonCode, Geneious Prime, R, Galaxy.

Région De Dijon, France

- Technical expertise in cellular imaging: BD FACS Aria II flow cytometer, Nikon A1-MP two-photon microscope, Continuum infrared microscope, Synchrotron-FTIR.- Selection of fluorescent probes for various applications: ROS detection, viability markers, intracellular activity, osmotic stress, membrane.- Handling of 2L, 3L, and 20L fermenters/bioreactors, as well as flasks.- Fluidized bed drying, lyophilization, biochemical and biophysical assays.- Data analysis using FlowJo, BD FACS Diva, Image J, Statistica, R, Unscrambler.- Poster and oral presentations at international scientific conferences.- Writing scientific manuscripts in English and French: articles, protocols, reports, patents, thesis.- Securing funding and grants for experimental research.- Supervision of seven interns at different levels and programs.- Four papers published.

Région De Dijon, France

- Practical work in industrial microbiology and genetics (32h).- Monitoring the diauxic growth of bacteria in bioreactors.- Enumeration of cells under the microscope and by a turbidimetric method.- Propagation of yeasts in fermenters (control and monitoring: pH, pO2, temperature, agitation, aeration).- Follow-up and advice to students in DUT biological engineering.

Région De Dijon, France

- Practical work in enzymatic engineering, biotechnologies and bioreactors (42h).- Optimization of enzyme production by Saccharomyces cerevisiae (control and monitoring: yield, sugar concentration, pH, agitation, temperature).- Installation of enzymatic reactors, free and immobilized, for the production of invert sugar.- Determination of kinetic and physico-chemical parameters (Vmax, Kpa, optimal temperature and pH, enzymatic assays).- Follow-up and advice to students in food engineering.

Natal, Rio Grande Do Norte, Brazil

- Development of a biological encapsulation process for fluorescent antioxidants.- Implementation of an extraction and quantification protocol by HPLC.- Technical expertise in cellular imaging: confocal and scanning electron microscopy.- Preparation of biological samples for microscopy (lyophilization, quality control).- Data analysis, modeling and construction of factorial experimental designs.- One published paper.

Aiserey, France

- Introduction to production line operations.- Mastery of mill safety and cleanliness standards.- Determination and systemic control of flour flows.

Dijon, Bourgogne-Franche-Comté, France

- Implementation of a protocol for rapid dehydration and rehydration of microorganisms.- Monitoring the stability of ferments after treatment.- Technical expertise in fluorescence microscopy and flow cytometry.- Handling of fluorescent probes: PI and FITC.- Data analysis, modeling, Statistica, BD FACS Diva.- One published paper.

Doctor Of Philosophy - Phd, Biotechnologies Agro-Alimentaires

Conservation and functional preservation of agri-food interest yeast http://www.theses.fr/2018UBFCK051

Msc In Chemical Engineering, Food And Biotechnological Processes

Fisetin microencapsulation in yeast cells (Saccharomyces cerevisiae) via non-lethal osmotic shock treatment.

Industrial Microbiology And Biotechnology, Food Biotechnology

New cell internalization strategies based on the application of transient osmotic flows between cells and their environment

Food Engineering

Bioethanol production using lignocellulosic residues from sugarcane industrial plants