Aaron Bradford Email & Phone Number

Acton, MA, US

Waltham, Massachusetts, United States

Cambridge, Massachusetts, United States

Boston, Massachusetts, United States

Weston, Massachusetts, United States

Greater Boston Area

Durham, New Hampshire

• Postdoctoral bench research and training in cell and molecular neurobiology laboratory.
• Performed team tasks characterizing common splice variations in mammalian neuronal calcium channels that cause behavioral changes in fear behaviors with applications to anxiety and PTSD.
• Developed projects combining proteomics of protein:protein interactions with cell and brain slice electrophysiology to characterize changes involving targets of interest.
• Collaborated on projects with teams at Broad Institute and Brown University studying similar topics in induced pluripotent stem cells and similar models.
• Optimized immunoprecipitation assays to isolate proteins of interest.
• Optimized electrophysiological recording protocols for thorough characterization of patched neurons in the pre-frontal cortex of mice.

Aberdeen Proving Grounds, MD

• Postdoctoral training program in molecular neurotoxicity laboratory.
• Characterized molecular effects of biological and chemical neurotoxicants and developed toxicant countermeasures in stem cell and ex vivo models.
• Developed reliable protocols to differentiate mouse embryonic stem cells into active, networked populations of neurons in vitro, with forays into same using human stem and pluripotent cells. Cell characterization with.
• Awarded competitive, independent, institutional research funding ($100,000) to develop transgenic embryonic stem cell lines for differentiation into neurons.
• Differentiated and characterized transgenic stem cell lines into neurons, including lines with genetically-encoded calcium indicators.
• Designed and transfected additional vectors to produce novel transgenic stem cells and differentiated neurons, including BAC and CRISPr constructs.

East Lansing, MI

• Successfully completed, defended and published neuroscience/neurotoxicology dissertation research “Involvement of the GABAA receptor in calcium-dependent toxicity of methylmercury in developing and aged cerebellum”
• Research techniques include tissue isolation, brain slicing, slice culture, fluorescent live cell and synaptic microscopy, concentration response assays, confocal and high speed microscopy, image processing, handling.
• Maintained and characterized only live colony of GABAA receptor alpha 6 subunit knockout mice, including genotyping techniques
• Pursued independent training in MATLAB and ImageJ for image processing and automation, presented results in major conference posters
• Wrote and revised detailed protocols and animal use documentation and grant preparation
• Implemented appropriate statistical analysis for many projects in the lab and regularly provided statistical support for new projects

Doctor Of Philosophy (Ph.D.), Biochemistry/Molecular Biology And Integrative Toxicology

Activities and Societies: Center for Integrative Toxicology Fellow Biochemistry Dept. Graduate Student Council Co-Chair Biochemistry Dept..

Ba, Chemistry, Sts