Joseph Abbott Email & Phone Number

Burlington, Ma, Us

Grow and maintain CHO cell cultures for use in experiments.Perform small scale and benchtop scale bioreactor experiments.Perform fed-batch and perfusion bioreactor experiments.Develop custom CHO cell culture medium to provide customers with improved performance and meet cost goals.Characterize and analyze competitor media products.Develop improved small scale bioreactor protocols to better represent larger scale bioreactors.Share and present results within and across teams at internal meetings.

Cambridge, Cambridgeshire, Gb

Design, synthesis, and validation of CRISPR/Cas9 guide RNAs for reagent orders and animal projects.Synthesis and validation of ZFN and Cas9 mRNA for animal projects.Maintain mammalian tissue cultures and execute transfection experiments.Create and perform qRTPCR assays of genetically modified tissues.Perform additional assays as required by team such as CRISPR/Cas9 off-target effect testing and short tandem repeat (STR) analysis.Assist genotyping team by generating positive controls and running genotyping assays as necessary.Cross trained with patient derived xenograft team in tumor dissociation and freezing.Prepare and quality control test donor plasmids.Responsible for creating, updating, and following SOPs.Create customer facing reports and prepare reagents for shipment.

St. Louis, Missouri, Us

Perform Illumina BeadArray microarray based fingerprinting assays which processes 35,000 samples annually.Operate and troubleshoot automated systems such as Tecan and Biomek liquid handling robots, and Real-Time PCR instruments and loading arms.Operate under ISO quality management system in high throughput production lab, follow and update SOPs and BPDs, and perform detailed record keeping.Active member of team that regularly sets data point production records and meets dynamic daily targets.Expert at all aspects of DNA/RNA extraction, TaqMan PCR, and proficient in data analysis.Optimized existing inventory system of primers, probes, and reagents resulting in a reduced rate of inventory disparities.Identified and implemented system improvements to real-time data handling system that resulted in streamlined and faster data analysis.Demonstrated ability to learn new techniques and to train other team members.Manage inventory of plant control tissue and seed. And maintain logs for patent law compliance.Prepare and run assay validation kits, controls, and assure purity of reference plant material.

Columbia, Mo, Us

Conducted independent research on MAP kinase signaling using molecular biology techniques.Designed and built custom plasmid vectors, transformed, grew, and isolated DNA from E. coli and Agrobacterium.Transformed Arabidopsis thaliana plants with cloned and modified genes creating sixteen genetically modified lines.Conducted forward and reverse genetic studies of Arabidopsis thaliana to increase understand of MAP kinase signaling.Studied molecular interactions in Nicotiana plants using fluorescence microscopy and protein immunoblot techniques. Maintained Nicotiana plants and performed experimental crosses. Executed proteomic study of developing soybeans using mass spectrometry analysis in order to better understand the tissue specific changes in their proteome during seed development. Performed luciferase assays as part of study of estrogen receptor response to xenoestrogens.Grew and maintained mammalian cell lines and aided in mouse colony care to provide model systems for the study of prostate cancer. Developed and executed protocol for enzyme purification and kinetic characterization as part of study of a pathway responsible for the synthesis of a quorum sensing molecule in Pseudomonas aeruginosa.Operated laboratory equipment and used techniques including HPLC, spectrophotometry, column chromatography and SDS-PAGE analysis as part of same Pseudomonas aeruginosa study. Optimized database for multidisciplinary team to help eliminate knowledge gaps for organic chemicals.Programmed software in Prolog computer language for maintenance of computer database and worked in a Linux software environment.