Abeba Demelash Ph.D. Email & Phone Number

San Francisco, Ca, Us

Rahway, New Jersey, Us

South San Francisco, California, Us

South San Francisco, California, Us

South San Francisco, California, Us

New York, Ny, Us

• Served as a council member who can consult on projects associated with the molecular diagnostic test for cancer research and histopathological platforms • Provided guidance and direction on biochemical, molecular and cellular biology study design to inform investment decisions• Advised clients on establishing appropriate research methodologies, resources and pricing to optimize profitability and meet business goals

Cleveland, Ohio, Us

• Led hypothesis-driven, chemotherapy resistance study. In this role, I was able to demonstrate strategic thinking by formulating research questions and link them to study designs to explore targetable molecules and their pathways in colon, melanoma and head & neck cancer, which led to a proposal and submission of over $ 1.25 million in NIH RO1 grant funding.• I have managed multiple projects with excellent project management and problem-solving skills. In this role, I was responsible for R&D sponsored projects (AbbVie oncology unite); developed and optimized IHC protocol and completed a systematic analysis of therapeutic bio-markers in more than 190 primary and metastatic colorectal cancer patient samples. • Evaluated potential therapeutic targets and their signaling pathways associated with the anti-tumor activity of small molecule drugs by using cell viability, proliferation, DNA damage, oxidative stress, senescence, apoptosis response on drug-sensitive and -resistance cancer cell lines.• Completed immunological staining of therapeutic targets in 20 melanoma, 27 head and neck squamous cell carcinoma HNSCC patient tissues.• Independently organized and maintained a molecular biology laboratory for over 2 years including monitoring chemicals, reagents, cleanliness, documentation; maintained budgets and negotiated supply costs; ensured the implementation of GLP, SOP regulations.• Collaborated with multidisciplinary teams, including pharmaceutical companies, clinicians and research scientist groups in a fast-paced research environment, contributed on a key project defining how cancer stem cell drive triple-negative breast cancer published on the scientific journal Nature Communication • Demonstrated excellent technical and problem‐solving skills by developing high throughput biochemical, molecular and cellular assays for the study of animal diseases ranging from genotyping to tissue pathological analysis.

Bethesda, Md, Us

PROJECT: Exploring the molecular pathology of lung carcinogenesis and differentiation.• Identified a novel mechanism of the CDK5, P35, and HASH cell signaling pathways in the initiation and progression of non-small cell lung cancer (NSCLC), leading to 2 peer-reviewed publications• Conceived and performed exploratory mouse model studies, including crossing and maintaining 6 colonies of transgenic mice, genotyping, injections, dissection, tissue processing, molecular histology of mouse diseases, immunohistochemistry, Laser capture microdissection (LCM), and data analysis• Worked in a dedicated and focused manner, preparing of 100 mouse DNA samples for genotyping in a week, performing of necropsies and tissue collection from 40 mice in a week, and stained 60 slides for histological diagnosis per week • Conceived and performed biochemical, molecular and cellular biology techniques including transfection, kinase assay, qPCR, migration, wound healing, immunofluorescence, co-immunoprecipitation to explore HASH signaling pathway responsible for the progression of lung cancer in vitro. • Effectively managed and controlled project budgets for 4 years

Göteborg, Västra Gotaland, Se

• Developed study design and conducted cell-based assays for data collection, accomplished several molecular and biochemical research objectives that define apoptotic signaling pathways in thyroid primary cells, neuronal cell differentiation/proliferation.• Technical expertise: Cell-based assays; primary cell culture; DNA and RNA Isolation and purification; DNA fragmentation; Western blotting; SDS-Page; Immunofluorescence; Confocal microscopy; Fluorescence microscopy; measuring trans-epithelial resistance; Mitochondrial morphology; Cytotoxicity (CellTiterGlo, LDH and MTT).

Göteborg, Västra Gotaland, Se

PROJECT: Understanding the apoptosis signaling pathway induced by oxidative stress and drugs in thyroid epithelial cells. The discoveries made in this project resulted in several awards and travel grants and 1 first authored peer-reviewed publication. Dissertation ISBN: 91-628-6249-9, Regulation of Apoptosis in the Thyroid Epithelium.• Skillfully performed cell-based assays using different approaches such as protein/DNA/RNA extractions, western blotting, cellular enzyme activity, and trans-epithelial electrical resistance measurements, immunofluorescent.• Developed study design and methods of data collection and analysis. Maintained detailed experimental records and laboratory notebook. • Presented weekly project progress reports at internal and external meetings, oral and poster presentations at national and international scientific meetings. Developed a genuine interest in and passion for oncology.

Ph.D. At Sahlgrenska University Hospital, Cell And Molecular Biology

Master'S Degree, Cellular And Molecular Biology

Bachelor'S Degree, Cell Biology