Nirupama Lal Email & Phone Number

Bangalore, Karnataka, India.

• Established required laboratory infrastructure • Served as Head of R&D for the design and development of translational research in the field of autoimmune disease like psoriasis and eczema, obesity and diabetes. • Played a key role in the designing project for the following molecules that are under the process of execution: • Agonist for Peroxisome Proliferator-activated receptors (PPAR) gamma,The antagonist for Dipeptidyl peptidase IV (DPP4/CD26), Farnesyl pyrophosphate synthetase (FPPS), platelet P2Y12 receptors, Phosphodiesterase 4.• Purification of Cell permeable peptides (CPP) and Antimicrobial peptides (AMP) from medicinal plants. • Assisted lab experiments in troubleshooting to ensure the proper functioning of the protocols. • Assisted in writing projects for various government fundings such as:o Oil and Gas PSU grant on Development of topical product from phytomolecules towards photoprotection against UV and skin cancer o Biotechnology Industry Research Assistance Council (BIRAC) for 'Finding innovative solutions to the growing threat of Antimicrobial resistance (AMR) by supporting the development of new antibiotics and alternatives to antibiotics', o Bill and Melinda Gates Foundation for 'New approaches for manufacturing gut microbial biotherapeutics'.• Trained in Schrodinger chemi-informatics, Bioinformatics (NGS), CRISPR CAS9 gene editing, Medicinal Chemistry, and Drug Discovery & Development India (MCADDI) 2019, Regulatory affairs an IP rights towards update in latest scientific technologies and regulations in the interest of company growth.

Ncr, Delhi, India

• Helped in the establishment of Section-8 Company.• Created the organization's mission and vision statements that would be utilized by all employees.• Developed and maintained positive relationships with employees.• Assisted with the creation of the marketing strategy and advertising initiatives that were used to promote the company to the public.• Organized discussion group (8+ members) focused to support the entrepreneurial talents in researchers or scientists towards the commercialization of their scientific aspirations.

Kanpur, Up.

• Worked directly with the department to achieve its goal.• Took four lectures per week during semester.• Areas taught: Cell biology, Microbiology, Ecology and Bioinformatics to M.Sc. postgraduate students.• Achieved the departmental objective by successful completion of the courses.

Hyderabad, India

• Awarded research grant and completed studies funded by academic groups. Independently designed and executed the project.• Established mitochondrial research lab in the institute.• Successful completion of the self-written project on 'Characterization of mitochondria associated senescence mechanism in obese mutant rat' through ICMR, New Delhi funding. • Compared the mitochondrial swelling in female WNIN/Obese (O) rats at different ages to that of WNIN-parental (W) and WNIN/Ob lean (L) rats as controls along with physical and biochemical parameters.• The obese rats were fed normal rat chow ad libitum and portrayed features of obesity such as higher body weight with hyperinsulinemia, which is similar to the preclinical /clinical features of obesity induced type 2 diabetes in humans.• The whole body dual energy X-ray absorptiometry (DXA) and biochemical analysis of fasting plasma were performed. • Liver mitochondria were isolated and subjected to Ca2+ -induced swelling study.• Obese rats of the designated WNIN/Obese (O) group showed decreased susceptibility to Ca2+ - induced swelling.• Additionally, it’s drastically worsened physical and biochemical indices compared to WNIN-parental (W) and WNIN/Ob lean (L) suggest that obesity is one of the factors which played active role in the alteration of the permeability of inner mitochondrial membrane.• Generated and published high-quality professional paper. • Presented poster at 5th Annual conference of Society for Mitochondrial Research and Medicine (SMRM), India.• Demonstrated mitochondrial potentials in diagnostics in institutional yearly seminar.

Hyderabad, Telangana, India

• Designed experiments to study genetics and epigenetics of obesogenecity.• Provided research support, case writing and data analysis, managed case submission process, edited, redrafted, and commented on working papers.• Drafted successful project proposal for funding from ICMR, New Delhi, India for Postdoctoral fellowship.• Co-authored 1 review on 'Genetics and epigenetic approach to Human Obesity'.• Managed creative projects from concept to completion.• Trained junior members of research team regarding molecular biology and troubleshooting of the experiments.• Conducted experiments, data analyses and lab seminars on a regular basis.• Distilled large amounts of information and produced reports, spreadsheets and documents outlining key details.

Hyderabad, Telangana, India

• Initiated the isolation and characterization of adult cardiac stem cell from perfused mice heart.• Set up equipment, organized inventory and maintained facilities.• Produced actionable information that improved the perfusion experiments.• Provided methodologies for the isolation of mono-nuclear cardiomyocytes.

Columbus, Ohio, Usa

• Established methods to detect and regulate mitochondrial programmed cell death.• Demonstrated active participation of complex-III of ETC in apoptosis.• Presented poster at 2008 VIIth Annual OSUMC Research Day on 'Association of complex-III of electron transport chain in apoptosis'.• Studied the 'Differential effects of non-steroidal anti-inflammatory drugs on mitochondrial dysfunction during oxidative stress' and got first author publication.• Produced and managed extensive data for fatty acid analysis under different conditions and provided recommendations to rescue the damage due to ischemic reperfusion in vitro.• Coordinated with other labs for aid in project completion.• Conducted cellular studies of mitochondria, which led to the development of other lab projects.• Developed prototypes for the study of mitochondria in the screening of the toxicity of the drugs. • Played key role in designing of the project by principal investigator for funding.

Columbus, Ohio, Usa

• Standardized protocols and conditions for the study of mitochondrial apoptosis.• Trained in studying mitochondrial biology and physiology at molecular level, isolation of mitochondria from heart, liver, brain and skeletal muscles, its permeability transition study through spectrophotometer, Ca2+ and Mg2+ estimations through atomic absorption spectroscopy (AAA), mitochondrial iPLA2 activity assay through GC, membrane potential measurement with tetraphenyl phosphonium (TPP+)-selective electrode, detection of Cytochrome C (Cyt C) release and reactive oxygen species (ROS) from mitochondria through HPLC and colorimetry/fluorescence spectrophotometer respectively.• Obtained online certificate for Institutional Animal Care and Use for the research purpose. • Supported Principal Investigator (PI) with daily research functions.• Produced data analysis reports for senior team members and PI.• Researched and updated all required materials needed for the lab.

Up, India

During my Ph.D. I used various DNA Fingerprinting techniques to study diversity among various species of aromatic grasses. I had been trained in various molecular biology techniques like from DNA/RNA isolation, qPCR, cloning, sequencing, hybridization, primer designing, molecular marker development etc. In brief I used to isolate DNA from different varieties and species of aromatic plants (Cymbopogon and Vetiveria) for doing PCR with arbitrary primers (designed inhouse) in order to study the degree of diversity among them through phylogenetic tree, component and Clustal W analysis. Further analysis was followed by cloning and sequencing of the selected co-migrating fragments through Sanger’s chain-termination method using fluorescent tagged dideoxynuleotides (ddNTP).

Uttarakhand, India

• Demonstrated a continued commitment to postgraduate teaching through full participation in the college community.• Taught Biophysics, Biochemistry, Molecular biology, Microbiology, Immunology in Biotechnology and Biomedical Sciences courses• Developed courses in line with the curriculum requirements of the Biotechnology Department.• Organized seminars and conferences related to molecular biology, biochemistry, microbiology, immunology etc. to create awareness in the students for the perspective research areas. • Established molecular biology and tissue culture lab and supervised and evaluated students' laboratory work.• Established departmental library for referencing the literature. • Administered and setup the question papers for midterm and final examinations. Also evaluated and graded students' performances.• Guided dissertation thesis of the postgraduate students.• Conducted the required course practicals.• Invigilated during the university exams.• Developed new process of teaching through seminars by the students, which resulted in marked improvements in their performances.• Supported Head of the Department with daily operational functions.• Responsible for creative designing of the prominent projects on profiling of local flora and fauna towards the diversity details of the country. • Instructed students in career development techniques including Molecular biology, Biochemistry and Microbiology. • Coached students on public speaking and presentation skills.

Lucknow, Up, India

• Identified species-specific molecular markers in both genera of Cymbopogon and Vetiveria zizanioides• Characterized both the genomes through RAPD and AFLP• Identified novel molecular marker showing sequence similarity with ndhD of evolutionary significance• Sequenced the marker fragment and submitted to Genebank as gbIEU871430.1• Presented results at departmental seminars and retreats to audiences of 20+ people• Generated high quality, professional papers for submission to scientific publications.• Participated actively in molecular biology workshops training for postgraduate students for their dissertations, university teachers, summer trainings, winter workshops etc. conducted by CSIR lab.• Oversaw studies, documented data and extrapolated the results.• Standardized protocols for DNA isolation, PCR, southern hybridization, cloning, autoradiography detection which led to the development of departmental research.• Assisted technical support to department in troubleshooting.• Provided drafting support to principal investigator and other scientists.

Lucknow, Up, India

• Learned and developed molecular biology skills through standardization of the techniques• Worked on molecular characterization of the medicinal and aromatic grasses.• Maintained high levels of confidentiality to ensure data quality and project research.• Performed various scientific duties on a daily basis, including autoclave, DNA isolation, PCR, media preparation, competent cell preparation, cloning and paper writing and presentation.• Developed and implemented laboratory protocols and modified when needed.

Lucknow, Uttar Pradesh, India

• Learned molecular biology skills like PCR, RAPD, gel electrophoresis etc., data compilation and literature search • Learned paper writing and presentations at seminars and conferences• Participated in organization of seminars and conferences/workshops• Learned molecular characterization of the medicinal and aromatic grasses.• Assisted seniors and principal investigators in their research project.

Ph.D., Lifesciences, Awarded

Activities and Societies: 1. National conference on Plant Biotechnology: Towards Strategic Agriculture and Drug Development, Lucknow.

M.Sc., Lifesciences, First Division

Activities and Societies: • 2nd Position in the University. • Successfully completed the coursework in in Molecular biology, Biochemistry.

B. Sc., Zoology, Botany & Chemistry, First Division

Activities and Societies: Done practicals and project work related to course curriculum. • Coursework in Zoology, Botany and Chemistry. •.