• Conducting routine diagnosis of SARS-CoV-2 from respiratory swabs using manual and automated procedures in a high-volume testing laboratory within the expected turnaround time.• Troubleshooting and optimizing the workflows, and routinely writing SOPs guiding the lab technicians.• Validation of new tests as well as different lot numbers of the reagents for routine clinical diagnosis.• Accessing patients’ records and reporting the lab test results according to APL’s best behavior policy.• Participation in multidirectional measures like LEAN-5S to maintain the quality of the laboratory testing.

• Planning and execution of multidirectional approaches for industrial scaling (GMP-complaint) of the developed diagnostic products through partnering with different stakeholders, including vendors, collaborators, manufacturers, and technicians.• Completing specialized laboratory experiments and preparing regulatory documents for FDA and Health Canada applications for emergency use authorization of diagnostic tests.• Training scientists, delivering instructions, and scientific recommendations to laboratories using the ILLUCIDx COVID-19 diagnostic testing solutions, which I have developed.• Conducted detailed evaluations of COVID-19 diagnostics tests from local and international competitors.• Utilized ENZYME quality management software (QMS) for keeping electronic laboratory records, manufacturing control, and implementing a quality management system.

• Designed, developed, and clinically validated a new simple high throughput test and a near-patient rapid diagnostic solution for COVID-19.• Translated federal and institutional biosafety guidelines into granular in-house aseptic standards for the day-to-day handling of infectious COVID-19 clinical specimens in BSL-2.• Guided the development of a microfluidic cartridge-based one-pot test to detect SARS-CoV2.• Conducted metagenomic sequencing of the respiratory specimens aiming at establishing a correlation between microbiome and SARS-CoV-2 infection.• Performed root-cause analysis and undertaken data-driven decisions to foster the continuous improvement of diagnostic procedures.• Mentored two graduate students by training, following up, providing feedback, and discussing problems.

• Cloned Bst DNA Polymerase and Lactate Dehydrogenase (ldh) genes from natural strains in multiple vectors and expressed them in different E. coli strains to escalate protein production.• Manipulated HEK293 cell lines to produce fluorescent proteins, such as GFPs and YFPs.• Conducted site-directed mutagenesis by PCR and CRISPR/Cas9-based techniques to study protein function and the effects of mutations on drug resistance.• Used chromatographic methods, such as Size Exclusion Chromatography (SEC) and Affinity Chromatography through manual columns and AKTA Pure instruments.• Designed, developed, and validated novel diagnostic methods for detecting malaria, resulting in three patents.• Designed a novel LAMP-based strategy for identifying single nucleotide polymorphism (SNP) in the gene of interest, and used the scheme to develop a diagnostic tool to detect K13 propeller mutation associated with artemisinin-resistant malaria.• Identified SNPs in the Kelch 13 propeller gene of P. falciparum by Sanger sequencing for deciphering anti-malarial drug resistance. • Maintained tissue culture, including human and parasite cell lines, for drug susceptibility and toxicity assays to discover new drugs and repurpose old remedies.• Examined the safety and efficacy of the novel and established anti-malarial drugs by in-vitro and ex-vivo assays for drug discovery and resistance monitoring.• Conducted/performed research to improve and simplify DNA/RNA extraction procedures to be used in different diagnostic platforms, such as LAMP, PCR, and NGS analysis.• Identified and solved problems through critical reviews of the literature, personal experience, receiving feedback from peers, and group discussions to finalize the study design and accomplish set objectives.• Developed project ideas and improved further by communicating with peers (co-workers, collaborators, and advisors) through presentations, group discussions, and formative feedback.

• Managed multiple projects by organizing information and resources, scheduling, distributing work, and prioritizing tasks to complete projects and deliver results.• Performed general laboratory administration, purchasing, detailed inventory management, and reconciliation of expenses through collaborating internally with multiple departments of the university.• Ensured a good laboratory practice (GLP) in place through keeping proper records, implementing health and safety regulations, and carrying out hazardous waste disposal according to the university procedures.• Regularly prepared common laboratory reagents, such as culture media, TE, PBS, running buffers, extraction buffers, stock drugs, and others, to maintain the smooth operation of the laboratory• Prepared different kinds of media for tissue and bacterial culture, and other necessary reagents for common use in different microbiological analysis• Ensured a clean and contamination-free working area by regularly cleaning the surfaces and equipment, autoclaving labware, and checking or calibrating equipment to produce consistent results.• Updated chemical inventory regularly through the university Chematix system

• Developed a new test based on Loop-Mediated Isothermal Amplification (LAMP) for a more effective diagnosis of malaria.• Performed third-party assessments using reference samples on rapid diagnostic tests for various infectious agents, including Flaviviruses, Plasmodium, Chikungunya, and HIV to monitor manufacturing quality and across different lots.• Validated ELISA, qPCR, and immunochromatography-based diagnostic panels for Plasmodium, Leishmania, Entamoeba, Cryptosporidium, Giardia, E. coli, Rotavirus, and others from several international companies using reference methods to support the development of the assays.• Detected Plasmodium infection in approximately 30,000 Anopheles mosquitos by ELISA and PCR to investigate malaria vector dynamics in Bangladesh.• Studied mosquito growth and dynamics in the field settings to study the environmental impact on disease transmission.• Kept a record of sample archives and experimental data through proper organization and entered information in the corresponding databases according to the recommended procedures.• Analyzed and interpreted experimental data with statistical tools such as PRISM and explained the study outcomes through manuscripts, technical reports, and presentation of the results in multiple settings.• Supervised three research assistants and five students within multiple research projects ensuring quality sample testing.• Maintained effective relationships through conference calls, emails, or direct consultation with external collaborators, such as Bill & Melinda Gates Foundation, Swiss Tropical Institute, and John Hopkins University to prepare grant applications, discuss project plans, and share technologies.