 Invented a patent-pending 96-well plate-based one-pot release and labeling technology for the rapid high-throughput preparation of O-glycans obtained from glycoproteins and analysis by LC-MS. This technology is the fastest, most sensitive, and high-throughput workflow for this analysis to date. Designed, invented, and synthesized a novel fluorescent dye as an orthogonal label for O-glycans. This new dye is to be implemented for LC-FLD-based applications in combination with optional mass spectrometry detection which fulfills the current market need for O-glycan analysis. Evaluated numerous release and labeling techniques to establish feasibility of a commercial O-glycan analysis kit. These methods include classical Carlson degradation, hydrazinolysis, and one-pot based release/labeling for analysis by LC-FLD, LC-FLD-MS, and/or LC-MS platforms. Assembled, managed, and directed a project team of one scientist and two research associates for the development of a commercial O-glycan analysis kit. Managed and directed bi-weekly team project meetings to ensure productivity towards the development of a commercial O-glycan analysis kit and evaluate the research performance and growth of each attendee. Attendees include two scientists and two research associates. Managed and administered training to two scientists and three research associates in the utilization of all Agilent instrumentation and associated software.  Supervised a scientist and two research associates on additional non-O-glycan projects which include intact protein analysis, BRAVO automation, sialic acid quantitation kit product requirements, instantQ purification, customer and marketing support, and general duties. Co-developed workflow procedures for Agilent’s AdvanceBio Gly-X APTS Express kit which resulted in its commercialization in 2019. Provided support to Agilent/ProZyme sales and marketing teams regarding customer-related questions regarding the ProZyme and Agilent portfolio.

 Played a key role in the acquisition of data needed for the commercialization of two ProZyme products: APTS Express and sialic acid quantitation kit. Collaborated with Richman Chemicals for the development and evaluation of dyes for improved glycan resolution in the ProZyme Gly-Q CE system. Independently and successfully site-prepped the Agilent Hayward site for a total of five Agilent instrumentation after its acquisition by Agilent. This involved preparation and installation of two Agilent LC/FLD systems in addition to an Agilent LC/MSD XT, Agilent LC/TOF MS, and Agilent LC/QTOF MS system. Site preparations include pump exhaust plumbing, nitrogen gas generators and associated plumbing, and electrical outlet installations. Transferred workflows and optimized the Agilent instrumentation to work in conjunction with the ProZyme product portfolio. Managed and administered LC and LC-MS training to research assistants and scientists.  Designed and directed research projects to a team consisting of three ProZyme research assistants. Successfully optimized and developed protocols for analysis of N- and O-glycans produced/labeled using both pre-existing and future ProZyme products on LC/FLD, LC-MS, and Gly-Q CE systems.  Developed workflows and protocols for the LC and Gly-Q CE analysis of glycans labeled using exploratory dyes containing “instant” chemistry.

Research advisor: Dr. Carlito B. Lebrilla Doctoral Thesis: Glycosidic Linkage Analysis by Liquid Chromatography-Mass Spectrometry Developed analytical and semi-preparative methods for liquid chromatography-based systems: HPLC, UHPLC, FlashLC, and NanoLC. Constructed analytical methods on various mass spectrometer instruments: Single Quad, Triple Quad, Q-Trap, LTQ-Orbitrap, TOF, and Q-TOF MS systems. Conducted, optimized, and performed analysis using electrospray and MALDI ionization sources. Analyzed samples using analytical instruments such as GC, UV-Vis, and NMR. Installed, site-prepped, repaired, and troubleshooted the aforementioned LC and MS instruments. Translated and performed scaled-up reactions from microliter to sub-kilogram 80-liter scale.  Administered an intensive glycan training program to scientists in Beijing, China. Developed and optimized inert organic chemical reactions. Integrated single-tube reactions and methods into 96-well plate formats for high-throughput applications. Constructed a multi-platform approach for the characterization of polysaccharides. Invented a high-throughput method for rapid glycosidic linkage analysis. Conducted data analysis and administered presentations for industry collaborators. Managed and trained numerous graduate, undergraduate, and visiting scholars on performing wet-lab techniques along with use, repair, and method development using LC-MS-based instruments.

Research advisors: Dr. Nader Pourmand & Dr. Bakthan SingaramBachelor Thesis: Attachment of Boronic Acid Onto Quartz Nanoporesfor Non-enzymatic Saccharide Detection Covalently attached boronic acid-containing polymers onto a quartz nanopipette for fabrication of a non-enzymatic saccharide sensor. Implemented dimethylacrylamide-based hydrogels containing various receptor groups for selective sensing of saccharides and/or protein-antibodies utilizing solid-state nano-pore technology. Synthesized, characterized, and applied polyelectrolytes for use as biosensors.University of California, Davis, September 2013Research advisor: Dr. Angelique Louie Assisted in the synthesis of Magnetic Resonance activated imaging compounds for use in staining cardiovascular diseases such as atherosclerosis.