Researcher/Editor (Senior Project)
Collaborated with Stryker and Michigan State University (MSU) across US and Ireland teams to assess the viability of using an ultrasonic aspirator in the Sonopet iQ medical instrument for resecting brain tissue. Participated in a two-team project focusing on testing and evaluating the viability of resected tissue from pig brains, simulating cancerous human-brain conditions. Held a key role in tracking project progress, editing team deliverables, and leading the creation of final reports and presentations, under an NDA.Key Responsibilities:• Served as team editor, managing documentation, ensuring adherence to project milestones, and organizing final paper and presentation materials.• Actively participated in testing sessions, refining procedures over multiple trial runs before the first successful attempt.• Performed laboratory work focused on determining tissue viability using DNA-based approaches and oxidative damage kits to measure 8-Hydroxy-2'-deoxyguanosine concentration as an indicator of cellular damage.• Led sample preparation by homogenizing brain tissue and utilizing centrifugation and mini-column techniques to isolate DNA components for viability analysis.• Evaluated 3 systems for final selection based on cost, ease of use/storage, extraction capabilities, time dedicated to gaining data.Systems Utilized During Project:• Cayman DNA/RNA Oxidation Damage ELISA Kit (High Sensitivity)• EZNA DNA Extraction Kit; created a matrix• CyQUANT MTT cell Viability Assay (3-(4,5-dimethylthiazol-2-yl)-2,5-dyphenyltetrazolium bromide) reagent• MS Office; specifically; Excel and V-Look Ups for data collection, MS Teams/Zoom for meetingsDevices Utilized During Project:• Thermo Scientific NanoDrop OneC UV-VIS Spectrophotometer• SpectraMax 384 Microplate• Centrifuge for DNA Extraction to isolate DNATraining/Certification:• MSU lab certification training for biological and toxic materials