Adam Wolin Email & Phone Number

Lexington, Massachusetts, United States

Cambridge, Massachusetts, United States

- Performed IND-enabling and preclinical efficacy research on humanized mouse models to evaluate LNP delivery methods, antibody-mediated conditioning regimens, and additional base-edited treatments for sickle cell disease- Worked across multiple teams to collaboratively design and implement studiesIn vivo Techniques:- Drug Delivery/Injections: IP, IV, Subcutaneous, Oral Gavage- Used aseptic technique in preparation of HSCs for IV transplant- Blood Collection: Submandibular & Cardiac puncture- Tissue Collection: Hind legs, Spleen & Liver- Tissue Processing: Flush bone marrow from hind legsEx vivo Assays:- Cell counting on NC-200, NC-250, and Advia 120- Tissue homogenization with Omni Prep- gDNA extraction for NGS- Mammalian Cell CultureTechnology:- Conducted analysis of experimental data in MS Excel and GraphPad Prism- Created and managed automated animal logs for colony management in Excel- Created graphs in Prism for internal presentations- Utilized and customized Studylog software for use in animal health tracking- Meticulously tracked and documented studies using Excel and Benchling ELN- Used Excel and Tableau to analyze data across multiple studies for an internal meta-analysisThis work resulted in multiple conference abstracts presented at ASGCT and ASH.

Manhasset, New York, United States

- Utilized multiple mouse models to investigate mechanisms of tau pathology driving dementia- Evaluated monoclonal-based immunotherapies to treat Alzheimer’s disease- Studied the effects of an anti-psychotic drug in a transgenic mouse model of psychosis- Maintained mouse colonies and managed animal inventories- Edited scientific papers to prepare for publication- Meticulous record-keeping and data organization- Coordinated sample storageIn vivo Techniques:• In vivo administration of anesthesia• Sacrificed up to 24 mice in a day• Collected and dissected brain and blood samples• Homogenized brain tissueEx vivo Methods:• Sectioned tissue on microtome• Performed immunocytochemical (ICC) staining• Performed Tau ELISAs and Luminex protein-concentration immunoassaysBehavioral Assays:• Pre-pulse inhibition of acoustic startle, using the SR-Lab Startle Response System• Barnes maze and open field, monitored with Ethovision XT camera-tracking softwareThis work resulted in 4 publications.

Baltimore, Maryland, United States

- Utilized rat model to test treatments for intra-ventricular hemorrhages in pre-term babies- Studied the connection between cerebral palsy and hyperalgesia in the same modelMethods:• ICV injections (free-hand)• Cognitive developmental testing• Touchscreen visual discrimination testing• Plantar testing• Heated tail immersion• Intramuscular administration of anesthesia• Transcardial perfusion fixation of whole animal• Extracted brain and spinal cord• Sectioned tissue on cryotome• Histochemical stainingThis work resulted in one publication.

Bethpage, New York, United States

- Built an insurance database in Microsoft Excel to help manage the credentialing of all the practices in the hospital networkI worked here for 2 consecutive summers (June-August) in 2016-2017

Baltimore, Maryland, United States

Collaborated with multiple writers to develop and edit scientific papers for publication in an annual journal, The Science in Society Review. This journal is published by The Triple Helix, a student-written run organziation with chapters at many universites.

Baltimore, Maryland, United States

- Prepared individuals to participate in an experiment on visual perception and attention- Consented the subjects and then explained the methods, risks, and purpose of the study to them

Mineola, New York, United States

- Prepared database of ICD 9 coded cases compared to ICD 10- Designed and implemented Practice Manager Handbook in Microsoft Publisher for staff to use as a reference tool

Mineola, New York, United States

- Assisted Dr. Mark Stecker in studying the performance of stroke alert teams.- Worked with multiple nurse practitioners to develop digital stroke logs and then to convert and manage seven years of stroke logs in Microsoft Excel- Analysis resulted in a publication

Mineola, New York, United States

- Assisted Dr. Thomas Jeitner in studying the causes of various neurological diseases including Alzheimer’s, Parkinson's and ALS- Analyzed samples of chlorodopamine and sulfurophane using flow-cytometry and UV spectroscopyto investigate mechanisms of oxidative stress and neurodegeneration

Bachelor’S Degree

Education record