Alison G. Email & Phone Number

California, United States

Marco Island, Florida, Us

Continuing with previous role in clients’ therapy at a new location

Marco Island, Florida, Us

Continued with previous role in clients’ therapy with BCAT certification

Marco Island, Florida, Us

Provided one-on-one clinic and home ABA therapy to clients (ranging from toddler to young adult in age) after the completion of rigorous on-site training, examination, and field evaluation; conducted lessons and managed behaviors as directed by supervisors with real-time data collection; attended clinics alongside each patient, their guardians, and members of their therapy team to discuss progress and program updatesInteracted with other therapists, supervisors, and patients’ families; exercised thorough and timely communication within the team to meet the demands of scheduling and session preparationMaintained HIPAA compliance at all times; responsible for closing the clinic at the end of assigned days, which included securing client informationReceived continuous training and regular drop-in supervision to learn from feedback and work toward achieving professional goals; completed Aggressive/Assaultive Behavior Management and CPR training

Independently grew and harvested 60-120L of bacteria per week for protein production; maintained lab/office for all in-house operations (labware, autoclave, stock solutions); ran SDS-PAGE gels, western blots (iBlot), and UV-Vis for production and analytical assay development; kept meticulously organized hardcopy lab notebooks and digital files; drafted protocols and SOPsCompleted 80-90% of Purchasing Department duties in Q4 2016; revamped purchasing procedure at the start of 2017 with improvements for organization and efficiency; collaborated with the VP of Finance & Business Development and the Controller for new purchase order and invoice system development; encouraged sustainability efforts (waste management, 5000 sq ft of rainforest conservation through a vendor promotion)Shadowed and received basic training of animal procedures (stereotaxic and stroke surgeries, injection, cheek bleed, sacrifice, brain removal, cryosectioning, brain matrix sectioning, tissue staining, calorimetric histochemistry)

South San Francisco, California, Us

Assembled and prepared equipment for production; operated manual and automated cleaning/sanitization and production processes; independently trained other technicians on fermentation operationsComplied with cGMP, SOP, and safety requirements; extensively documented and reviewed the use of all equipment, data, and participantsTroubleshot equipment and process problems both within assigned team for the shift as well as with other departments (MSAT, Automation, Facilities, CHO); recognized non-routine issues and suggested solutions to meet production demands; promoted organization and inspection readiness as an active member of the 5S teamNotable relevant skills and duties include, but are not limited to:GMP; cGMP; GSP; GMP documentation; following SOPs; location- and task-specific proper gowning; safe work habits; safety suggestions; best practice; 5S; 5S audit; 5S team; small to large scale production; aseptic techniques; cleaning and use of Laminar Flow Hood; cleaning and use of incubated flask shaker; preparation of post-sterile additions; manual and automated CIP of portable and fixed tanks; manual and automated SIP of portable and fixed tanks (media preparation, steaming, post-sterile additions); manual steam sanitization of portable filter housings; samples and readings; large scale production monitoring; aseptic samples; buffer preparation; filter integrity test (Sartocheck); pallet jack use; glucose batching; Yokogawa recorder monitoring; pH meter standardization; dO2 meter standardization; chromatography skid operations; (wall-to-wall) cycle count; work notification submission; FDA inspection readiness; MES; IP21; Aspen Process Explorer; Labware LIMS; SAP; fermentation operations training

(Please see "The Science and Engineering Mentoring Outreach (SEMO) Program" in the "Organizations" section.)Notes:This position is related to "Teaching Assistant for 'STEM Education and Social Justice' (BIOL 188) / Santa Clara University" in this work experience section.

Primarily assisted my mentor in his graduate school research focusing on the proteasome through smaller independent projects involving cloning, Western blot, and tissue cultureProduced a properly localizing green fluorescent protein and nuclear retention signal construct which the lab could continue to use in the futureKept an organized digital lab notebook; maintained tidiness in a shared bench area; aided the general upkeep of the lab and plasmid databaseNotable relevant skills and duties:PCR; agarose/SDS-PAGE gel electrophoresis; aseptic techniques; plating; plasmid mini-/midiprep; making overnight cultures; glycerol stock preparation; molecular cloning from start to finish (incl. primer design, digestion, ligation, transformation, gel extraction); SOE PCR; quick change; PCR purification; colony PCR with Sapphire; cell/tissue culture techniques (incl. maintaining/splitting, plating, transfecting, harvesting); Bradford assay; Western blot; ethanol precipitation of nucleic acids; limited experience in BAC mutagenesis and pulsed-field gel electrophoresis; NanoDrop; ApE

Santa Clara, Ca, Us

Helped design a companion course for the SEMO Program at Santa Clara University, titled "STEM Education and Social Justice" (BIOL 188) to examine the issues and challenges surrounding STEM education in U.S. schools, with a focus on high-risk and/or disadvantaged high schools in the San Francisco Bay Area, CAAssisted the professor with syllabus writing, getting Experiential Learning for Social Justice (ELSJ) University Core requirement approval, and arranging mentoring visits to local high schoolsCourse description:"This course will examine the issues and challenges surrounding science, technology, engineering, and mathematics (STEM) education in public science schools in the US. Students will explore these topics while taking part in the Science and Engineering Mentoring Outreach (SEMO) program mentoring high school students, while receiving instruction from a diverse range of experts on STEM education." (written by Dr. Katy Korsmeyer)Notes:This position is related to "Co-founder; Creative Designs and Administrative Director / The Science and Engineering Mentoring Outreach (SEMO) Program" in this work experience section, and "The Science and Engineering Mentoring Outreach (SEMO) Program" in the "Organizations" section.

Us

Worked on the production farm and greenhouse to provide for the farm stand, local restaurants, fundraisers, and soup kitchens; reinforced knowledge of sustainable growing practicesHelped manage education garden and directed volunteers in harvesting, compost turning, planting, etc.

Research in teen health-related issues and wrote a comprehensive report on data, findings, recommendations, etc.Presention to faculty; Alameda Family Services Executive Board; Alameda Collaborative for Children, Youth, and Families; Alameda Unified School District School BoardAdvocated and petitioned for teen rights in the health center for high schools of the Alameda Unified School DistrictPresented to the Alameda County Board of Supervisors in Dec 2006