Angela Guo Email and Phone Number

Scientist in the Epigenetics Applications- Developed and validated the CUT&Tag assay, producing one CUT&Tag kit with a detailed protocol, three associated products, 32 validated antibodies, and data images for customer reference.- Coordinate with NGS and Bioinformatics groups to optimize NGS analysis as a step of the antibody development and validation pipeline- Analyzed over 2000 NGS ChIP-seq, CUT&RUN, and CUT&Tag samples- Provided scientific support for academic and industry… Show more Scientist in the Epigenetics Applications- Developed and validated the CUT&Tag assay, producing one CUT&Tag kit with a detailed protocol, three associated products, 32 validated antibodies, and data images for customer reference.- Coordinate with NGS and Bioinformatics groups to optimize NGS analysis as a step of the antibody development and validation pipeline- Analyzed over 2000 NGS ChIP-seq, CUT&RUN, and CUT&Tag samples- Provided scientific support for academic and industry customers regarding chromatin-based assays through Salesforce and video calls - Engaged with KOLs in epigenetics through conference attendance with poster presentations (AACR, Gordon conference), on-sight meetings (5 per year), and presented multiple times at the annual CST company meeting.- Mentored interns through the CST internship program and trained team members to run basic bioinformatics commands on bash and R for NGS analysis. Show less

Mentor: David Lombard- Elucidated mechanisms by which SIRT5 protects against pressure overload-induced heart failure in mice using techniques including RNA-sequencing, metabolomics, western blotting, and statistical analysis (published).- Identified epigenetic consequences of cadmium toxicity in tissue culture and mouse models through a multi-omics approach including RNA-seq, ChIP-seq, and metabolomics (work in progress).- Defined the molecular mechanism of age-related… Show more Mentor: David Lombard- Elucidated mechanisms by which SIRT5 protects against pressure overload-induced heart failure in mice using techniques including RNA-sequencing, metabolomics, western blotting, and statistical analysis (published).- Identified epigenetic consequences of cadmium toxicity in tissue culture and mouse models through a multi-omics approach including RNA-seq, ChIP-seq, and metabolomics (work in progress).- Defined the molecular mechanism of age-related heterochromatic dysregulation through chromatin immunoprecipitation in young and old mouse heart tissue (work in progress).- Generated CRISPR-mediated knock-out cell lines for all lab projects by creating plasmid constructs, making virus for transduction, and verifying knock-out status.- Developed and established an RNA-sequencing pipeline for the lab using the bash, R, and web-based gene ontology tools, analyzing data for 9 projects, leading to 3 current publications. Show less

Biolchem 452: advanced biochemistry course covering of selected topics in Molecular Biology, Genetics, Cell Biology, and Signal Transduction.

Advisor: Brian Strahl, Scott Rothbart- Characterized a novel post-translational modification-dependent protein-protein interaction between the histone demethylase JMJD2a and the tumor suppressor p53 using peptide pulldowns and western blotting.- Determined the specificity of antibodies raised to recognize histone post-translational modifications using peptide microarrays (publication).- Contributed to 3 publications by generating plasmid construction using traditional and Gibson… Show more Advisor: Brian Strahl, Scott Rothbart- Characterized a novel post-translational modification-dependent protein-protein interaction between the histone demethylase JMJD2a and the tumor suppressor p53 using peptide pulldowns and western blotting.- Determined the specificity of antibodies raised to recognize histone post-translational modifications using peptide microarrays (publication).- Contributed to 3 publications by generating plasmid construction using traditional and Gibson cloning techniques and purifying the proteins used in these studies. Show less

Biol 535: Techniques in Molecular BiologyManaging Professor: Darrel Stafford