Aida Trice, M.Sc. Email and Phone Number

-Custom Cell Culture Media design and development for RUO and GMP Manufacturing - CDMO Process Development Engineer-Client consultations involving project designs, troubleshooting of formulations and process builds - RUO and GMP productions- Planning, drafting, and fi nal GMP/RUO execution for fi nal commercial product-Routine testing support, process validations, qualifi cation direct support for products with QC-Initiation of qualification protocols and reports for RUO and GMP production executed reports and validation studies for custom and standard GMP formulations-Optimized cGMP process for reduction of time, cost, and labour, while maintaining product quality-Implementation of scalable platform designs optimized to automation-New product tech transfers to MFG Risk Management and Risk Evaluation (RMRE's) for all incoming material for commercial or research production Provided PFMEA's for processes for tech transfers to cleanroom spaces-Development of tech transfer programs and staff training for MSAT/PD inclusive of tech transfers-Creation of technical documentations, SOP, Validations (CAPA's and V&V), and Br for new and updated processes

-Led CMO Process Development as Sr. Research Associate in a biopharmaceutical company, focusing on cancer immunotherapies using iPSCs, NK, T-Cells, CAR-T-Expert in cell differentiation culture processes in commercial cleanrooms; investigated cGMP MGF process deviations-Authored SOPs, BRs, and OJTs; spearheaded training programs, including new hires, refresher courses, re-qualifi cations, and tech transfers-Specialized in characterizing and differentiating iPSC in various scales; utilized antibodies, cytokines for media formulation-Played a pivotal role in guiding CMC Pilot runs for IND submissions; collaborated across departments to optimize upstream/downstream experiments-Conducted comprehensive data analysis using JMP/GraphPad Prism; performed cell, protein assays, and multi-parameter flow cytometry.-Supported and conducted additional molecular assays, including Western Blotting

Masters Candidate for Dr. Schiavo at University College London Institute of Neurology, Neurodegenerative Disorders in Motor Neurons-Performed mouse dissections using SOD1 and WT animal model for investigation of neuronal activity within the neuromuscular junctions-Tissue and Spinal cord collection for sectioning prep using cryostat, and immunohistochemical staining assays-Design and execution of immunohistochemical assays (staining protocols) for the investigation light intensity captured between Disease vs. WT using LSM-Presented critical data and analysis and current research journals for Department of Neurology core meetings-Executed various assays in strict adherence to the radiation protocols-Handling of mouse genetic models of disease for prep of injections and ID-tagging-Aided and performed ddPCR for biodistribuition studies

-Proposed, redesigned, and created SOP/Documentation for GMP production-Implementation of platforms such as 36-layer hyper-stacks to for scalability and streamlining product-Organized demos on biotech equipment and data analyzing programs for GMP process useCryopreservation of cell samples for quality testing or cell banking-Initiated, completed, and fi led experiment reports and studies-Statistical analysis on experimental and procedural studies using JMP to predict feasibility and productivity for future studies-Troubleshooting and investigations of suboptimal cell performances occurring in MFG

-MACSQuant fl ow cytometer for antibody expression in NK cells post-test for tumor performance-Designed, developed, and performed ELISA protocols for trace element testing-Conducted ADCC, Cytotoxicity Assays, wester blotting of various tumor cell lines-Cell banking including cryopreservation of cell lines-Performed functional and stability tests NK cell lines and tumor human cell lines-Set up and maintained K-Sep system 400 and XDR for large scale harvests from Xuri 25-50L bioreactors-IND submissions

-Cell culture and antibody concentration of hybridoma and CHO cell lines-Thawed, adapted and expanded cells-Troubleshooting of cell lines with poor performance via formulation adjustments-Performed qPCR to detect mycoplasma from post purifi ed reagents and proteins-Experienced with Wave 20/50 Bioreactors 10L-200L, fl asks and roller bottles-Performed ELISA assays to ensure correct specificities of clones-Used and maintained cell biochemical analyzers NOVA BioProfile 400

Immune Mechanisms and the Impact of Estrogen Inducing Racial Disparities in Prostate Cancer• Tissue culture of various human prostate cancer cell lines• Treated cell lines with gamma interferon and estrogen to test disparities within various genes• Performed RNA extractions from harvested cells to collected data for qPCR via gene amplification.• Performed cDNA syntheses from tissue culture to prep for PCR analysis• Cytotoxicity testing for data comparisons• Designed individual project treating cells with or w/o beta-estradiol after serum starvation for 24 hours