Aishwariya Raksha Siddharthan Email & Phone Number

Boston, Ma, Us

Lexington, Massachusetts, United States

• Determining relevant TGF-beta receptor and ligand expression alterations in both bone marrow stromal cells and hematopoietic cells in wild type compared to hematopoietic disease cells.• Overexpressing key TGF-beta ligands and genes of interest via lentiviral vectors to assess hematopoietic and bone marrow stromal cell proliferation and differentiation alterations.• Assessing microenvironmental changes that occur during hematopoietic disease progression.• Applying an array of downstream readouts including qPCR, ELISAs, Western blotting, and lentiviral transduction to assist in developing and supporting the hypothesis.

North Dartmouth, Massachusetts, United States

• Conducted research on A375 cells to investigate the efficiency of PLGA microparticles as a drug delivery system.• Primarily involved in culturing and maintaining A375 cells, ensuring optimal conditions for experimentation• Actively performed western blots, flow cytometry and qPCR to assess gene markers expressed during cell death, contributing to a comprehensive understanding of the cellular response to PLGA with Dacarbazine• Actively engaged in bridging the realms of drug delivery and cellular biology, bringing together expertise in PLGA as a drug delivery system and its impact on cellular responses.• This interdisciplinary approach aims to elucidate the intricate interplay between the delivery system and cellular processes.

Thanjavur, Tamil Nadu, India

• Engineered a robust detection model leveraging motion and facial features associated with epilepsy• Applied advanced algorithms to analyze EEG data and video inputs, contributing to enhanced accuracy in seizure detection• Developed an intuitive intimation model integrated with the IFTTT (If This Then That) application• This innovative model allowed for real-time notification and alerts to relevant stakeholders upon seizure detection, improving response times and patient careThis project equipped me with hands-on experience in interdisciplinary research at the intersection of neuroscience and technology, fostering skills in data analysis, algorithm development, and the practical application of research findings for improved healthcare outcomes.

Thanjavur, Tamil Nadu, India

• Spearheaded a project centered on the isolation of phages targeting Salmonella Typhimurium, advancing the exploration of bacteriophage therapy as a potential treatment avenue• Executed a meticulous comparative genomic analysis, leveraging previously identified prophages against Salmonella Typhimurium • Utilized tools such as BLAST for sequence alignment, GeneMark for gene prediction, and PHASTER for phage annotation, gaining insights into genetic variations within prophages against Salmonella TyphimuriumThis project honed my skills in microbiology research, particularly in the context of phage therapy and genomic analysis, and provided a valuable contribution to ongoing efforts in combating Salmonella Typhimurium infections

Quebec, Canada

• Engaged in groundbreaking research focused on "Novel Therapeutics to Combat Cancer Progression,"• Conducted cell culture experiments with NMuMG cells, inducing Epithelial–Mesenchymal Transition (EMT) through Applied meticulous techniques to ensure the reproducibility and reliability of cellular models simulating cancer progression.• Investigated the impact of EPB41L5 on EMT-induced cells. Administered EPB41L5 treatment and assessed effects through Western Blot analysis, providing crucial insights into the potential therapeutic efficacy of EPB41L5 in inhibiting cancer progression.• Explored strategies to inhibit the oligomerization of EPB41L5, a key protein associated with cancer progression. Implemented experimental protocols to modulate EPB41L5 interactions, contributing to the development of targeted therapeutic approaches.• Investigated the efficacy of SUPER1, a potential anti-metastatic drug for breast cancer. Conducted experiments at varying concentrations to assess its impact on cancer cell metastasis, providing valuable data for ongoing drug development initiatives.This internship provided a hands-on experience in cutting-edge cancer research, allowing me to contribute to the development of potential therapeutics targeting cancer progression and metastasis.

Chennai, Tamil Nadu, India

• Gained experience in various departments that include Hematology, Histopathology and Cytology, Clinical Biochemistry, Stem cells, Sample Collection and Blood Bank and Nuclear Medicine • Received training in the operation of different equipment, such as the Coagulation Analyzer and CBC Analyzer, Cryostat, Tissue Processor, and the Multistainer.• Performed the techniques of Immunocytochemistry that include culturing the cells, Immunofluorescence Staining, Confocal Microscopy and the Image Analysis

Chennai, Tamil Nadu, India

• Engaged in a hands-on training program at Pfizer Healthcare Private Limited, gaining valuable experience with industrial equipment, including various bioreactors and calorimeters. • Acquired practical experience and proficiency in operating diverse industrial equipment, with a focus on bioreactors and calorimeters. This hands-on training provided insights into the application of these instruments in pharmaceutical processes.• Conducted an extensive literature study on Critical Process Parameters (CPPs) associated with the industrial equipment. Explored the nuances of these parameters and their significance in ensuring the quality and efficiency of pharmaceutical processes.• Investigated and documented hazards associated with the operation of industrial equipment, contributing to a comprehensive understanding of safety protocols and risk management in a pharmaceutical manufacturing environment.This internship at Pfizer Healthcare Private Limited enhanced my technical skills in handling industrial equipment and deepened my knowledge of critical process parameters and safety considerations in pharmaceutical manufacturing. It provided a valuable insight into the practical aspects of the industry, contributing to my professional development in the field.

• Conducted meticulous aseptic maintenance and expansion procedures for diverse stem cell lines, ensuring their optimal viability. This hands-on experience reinforced proficiency in maintaining cellular cultures to rigorous standards.• Collaborated seamlessly with cross-functional teams, fostering a dynamic exchange of ideas and insights. Presented research findings and contributed to ongoing projects, actively participating in the collective pursuit of advancements in stem cell research.• Demonstrated proficiency in essential laboratory techniques, including accurate cell counting and viability assays. These skills were crucial in maintaining precise control over experimental conditions and ensuring the reliability of research outcomes.• Operated a variety of laboratory equipment with precision, showcasing competence in the use of sophisticated instruments integral to stem cell research protocols.The internship at Avigen Biotech Private Limited provided a comprehensive exposure to the intricacies of stem cell research and laboratory operations. The collaborative environment enabled me to contribute meaningfully to ongoing projects while further developing my skills in aseptic techniques, cell culture maintenance, and laboratory procedures.

Chennai, Tamil Nadu, India

• Conducted detailed examinations of karyotypes associated with Down Syndrome, identifying and documenting chromosomal abnormalities. This involved using microscopic analysis and image capture software to precisely identify and catalog genetic variations.• Delved into the karyotypes of individuals with Klinefelter Syndrome, aiming to comprehend the genetic basis of this condition. Applied cytogenetic techniques to recognize the extra X chromosome in affected individuals and contributed to the institute's knowledge base on this genetic disorder.• Explored the characteristics of the Philadelphia Chromosome, a critical marker in various hematological malignancies, particularly in chronic myeloid leukemia (CML). Employed karyotyping techniques to discern the reciprocal translocation involving chromosomes 9 and 22, adding to the institute's understanding of cancer genetics.This internship not only equipped me with hands-on experience in karyotyping techniques but also deepened my understanding of the genetic basis of conditions such as Down Syndrome, Klinefelter Syndrome, and the implications of the Philadelphia Chromosome in hematological disorders.

Master'S Degree, Bioengineering And Biomedical Engineering

B.Tech, Bioengineering And Biomedical Engineering