Develop the next generation of liquid biopsy assays and products

NGS based assays • Protein aggregation • Mammalian cell culture • Rare diseases • Mentoring • Principal InvestigatorDeveloped genome wide sequencing assays to study rare disease alleles.- Led cross-functional collaboration of a multi-laboratory team across two separate institutions: Stanford University and MD Anderson Cancer Center.- Designed a NGS based assay to investigate altered protein conformations.- Leveraged above assay to query human ORF collection and Mendelian rare disease collection.- Designed secondary flow cytometry based approaches for hits validation and testing.- Designed mammalian cell lines for hits validation.- Acquired competitive research support ($180k) from National Institutes of Health as Principal Investigator.High-throughput epigenomics of chromatin remodeling complexes.- Mentored a team of graduate students to conduct epigenomics on chromatin remodeling complexes.- Developed and designed high throughput liquid handling pipelines for growth phenotyping assays of yeast strains.- Designed novel transformation techniques for protein aggregates.- Drafted one peer-reviewed research article (Cell).Think tank planner, Jarosz research group.- Planned biweekly and monthly meetings for 15 laboratory members for discussing research plans and updates.- Moderated meeting discussions, summarized action items and follow-up.

RNA Biology • Extramural research funding • Epigenomics • Intrinsically disordered proteins • Gene expressionDiscovered a novel class of protein-based epigenetic elements. - Mentored a team of two students to investigate a novel class of non-amyloid prions.- Developed novel protein purification strategies and biochemical assays for intrinsically disordered RNA binding proteins.- Designed live cell microscopy methods, high-throughput Gateway® cloning vectors and yeast strains (homology directed repair, CRISPR/Cas9).- Coordinated operations with third party CROs for NGS library preparations.- Developed bioinformatics pipelines for RNA sequencing analyses (R, Python), evolutionary protein disorder analyses, quantification of microscopy and flow cytometry data.- Delivered oral presentations at national and international scientific conferences and seminars.- Acquired competitive research support ($208k) from prestigious cancer research foundation.- Drafted and finalized two peer-reviewed research articles and one review article.Design of a massively parallel transcriptomic assay to quantify RNA-protein interactions.- Led cross-functional collaboration of a multi-laboratory team across departments.- Designed, developed and validated a novel workflow to assay RNA-protein interactions with high biophysical precision at transcriptome-wide scale.- Secured competitive seed funding ($25k), conducted key experiments and led regular team meetings.- Drafted and delivered multiple technical presentations (posters, talks) at prestigious scientific conferences.- Composed and finalized manuscript for peer-reviewed publication.

Protein engineering • Mechanistic enzymology • RNA biology • Structural biology • Molecular geneticsCharacterized suite of nucleic acid processing enzymes that read, write and edit DNA and RNAs. - Authored 8 peer-reviewed publications (6 as first author) in prestigious journals (PNAS, Cell Reports, NAR, JBC).- Used standard molecular biology techniques and protein engineering to express and purify protein complexes.- Developed pre-steady state kinetic assays (radio- and fluorophore-labeled probes) for nucleic acid processing enzymes.- Drove several collaborative structural biology projects to screen, optimize, crystallize and solve structures of nucleic acid-protein complexes (molecular replacement, SeMet experimental phasing).- Designed novel end-modified nucleic acid substrates for enzymology and crystallography applications.- Developed molecular genetic assays to investigate novel eukaryal anticodon targeting toxins.