Protein Purification Lab Manager:Leading a team of technical and scientific personnelManaging the protein production facilityDeveloping and refining protein purification processesConducting large-scale purification (IMAC, IEX, HIC, SEC)Validating proteins through physical and functional assaysDocumenting processes using BenchlingAssay development

Led a project to unravel the interaction between human Ca2+-transporting ATPase and alpha-synuclein, with findings published in EMBO Journal: https://www.embopress.org/doi/full/10.15252/embj.2022111122Key Techniques:Protein expression (E. coli, S. cerevisiae)Protein purification (soluble, transmembrane)Immunocharacterization (WB, co-IP)Enzyme kineticsBiophysics (MST, CD, SAXS)Cryo-electron microscopyProtein interaction & structure/function analysisProtein engineering & mutagenesis

Enzymatic assays optimization

Membrane protein production & purification (affinity and size exclusion chromatography with ÅKTA systems)Molecular cloning, protein engineering, and mutagenesis Comprehensive protein characterization

Developed a workflow for expressing, harvesting, and purifying human plasma membrane Ca2+-ATPases for functional and structural studies.Molecular cloning, E. coli & S. cerevisiae cultivationProtein production, purification, and enzymatic assaysCell fractionationBasic protein characterization (solubility, stability, SDS-PAGE, WB, in-gel fluorescence, proteolytic digestion)

Contributed to research on energetic metabolism, cell homeostasis, and calcium transport while teaching biochemistry to medical students.Skills:Mammalian cell culture (GH3, PC12), transfection, gene silencing, cell differentiationDNA/RNA isolation, PCR (including RT-PCR and qPCR)Cell-based assays (viability, proliferation, Ca2+, ATP, glutathione), enzymatic assaysSpectrophotometry, fluorimetry, bioluminescenceLight and fluorescence microscopyCell labeling for flow cytometry and immunocytochemistry

As a member of the pilot team of technological scouts, I conducted interviews with local academic researchers and assessed the commercial potential of their innovations.