Albert Hung Email & Phone Number

Berkeley, Ca, Us

New York, New York, United States

• Pursuing PhD degree in Biomedical Sciences under the mentorship of Dr. Domenico Accili and Dr. Eunhee Choi at Vagelos College of Physicians and Surgeons, Columbia University• Exploring the pharmacological properties of S597 insulin receptor agonist and the mechanism through which S597 induced bias signaling in different tissues• Exploring whether S597 alleviates severe insulin resistance in D707A insulin receptor knock in mice

Taipei City, Taiwan

Dr. Soo-Chen Cheng Laboratory• Supporting efforts to characterize the function of MSL5 and NPL3 in RNA splicing in yeast• Generated MSL5 suppressed yeast strain through glucose repression of GAL1 promoter• Developing NPL3 knockout yeast strain through UTR double homologous recombination• Designed plasmids for transformation and integration into yeast through molecular cloning• Skills: Molecular Cloning (PCR, Restriction enzyme digestion, E. coli culture, Plasmid extraction and isolation), Yeast transformation and integration, Yeast culture, Western blot

San Francisco, California

Dr. Tien Peng Laboratory• Described the connection between Hedgehog signaling pathway and pulmonary inflammation• Assisted in breeding heterozygous Hedgehog interacting protein (HHIP) knockout mice and demonstrated that suppression of HHIP expression leads to pulmonary inflammation in vivo• Developed NK cell-based assay to demonstrate that HHIP inhibits NK cell proliferation • Skills: Mouse genotyping (PCR tail biopsy), Mouse lung dissection, Immunostaining, H&E staining, NK cell culture, RT-qPCR, FACS, Lung organelle culture

South San Francisco, California

• Probed the IR residue that was previously found critical for IR activation• Developed stable cell lines that expressed specifically mutated IR to lower background signals• Mutated the residue to amino acids other than alanine to understand the importance of the residue for IR activation• Applied MOE and MD analysis to generate hypotheses on poses favorable for receptor activation and to correlate my in vitro findings at the atomic level• Revealed a potential site for allosteric regulation on the insulin receptor• Skills: Computational Biology (MD analysis), Cell culture, ELISA

South San Francisco, California

• Identified key amino acid residues of the insulin receptor (IR) that are critical for activation of downstream signaling pathways through alanine mutagenesis assay• Optimized and developed alanine mutagenesis analysis on the insulin receptor• Skills: Cell culture, Lipofectamine transient transfection, Western blot, ELISA

Greater Los Angeles Area

• Assisted in characterizing dozens of novel compounds found in induced pluripotent stem cell (iPSC) using cell fractionation and mass spectrometry • Identified novel glycylated sugar alcohols in iPSCs that protect microRNA from degradation (Chang-Lin, Samantha, Hung, Albert et al. Nucleic acids research vol. 44,10 (2016): 4894-906)• Developed RT-qPCR assay to demonstrate the RNA protecting property of the sugar alcohols• Skills: PCR, RT-qPCR, Western blot, Cell fractionation, Gel electrophoresis, Northern blot

Taipei City, Taiwan

• Proposed using microRNA-302 (miR-302) to probe the connection between type two diabetes and obesity based on known information on the inhibition of lipid drop formation by miR-302 in adipose tissue• Took a gap year after my sophomore year in high school to dedicate full-time on research• Demonstrated that miR-302 alleviates diabetic symptoms of high blood glucose level and insulin resistance in vivo by measuring blood glucose level daily and performing glucose tolerance test• Dissected hepatic tissues for immunostaining to identify pathways affected by miR-302 • Developed hepatocyte-based assay to demonstrate increased phosphorylation of Akt• Skills: Mouse handling , Immunostaining, Mammalian cell culture

Doctor Of Philosophy - Phd, Biological And Biomedical Sciences

Columbia University, Vagelos College of Physicians and Surgeons

Bachelor'S Degree, Molecular And Cell Biology, 4.0

Graduated with the Highest Distinction in General Scholarship

High School Diploma

High School Diploma

High School Diploma