Aldo Castillo

Aldo Castillo Email and Phone Number

Associate Scientist @ Gilead Sciences
Aldo Castillo's Location
Los Angeles, California, United States, United States
Aldo Castillo's Contact Details

Aldo Castillo personal email

n/a
About Aldo Castillo

Experience Senior Research Associate with a demonstrated history of working in the Biotech Industry as well as in the Academia. Skilled in Cell Culture, Cell and Molecular Biology, In vivo and Immuno assays

Aldo Castillo's Current Company Details
Gilead Sciences

Gilead Sciences

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Associate Scientist
Website:
gilead.com
Aldo Castillo Work Experience Details
  • Gilead Sciences
    Associate Scientist
    Gilead Sciences May 2022 - Present
    Foster City, Ca, Us
  • Staidson Biopharma Inc
    Senior Research Associate
    Staidson Biopharma Inc Jan 2020 - Aug 2021
  • Staidson Biopharma
    Research Associate Ii. Antibody Discovery And Development
    Staidson Biopharma Oct 2018 - Aug 2021
    My role as a team member of the Antibody discovery group includes mice immunization, B-cell isolation. Sera titration, cell fusion, selection and expanding mAB. ELISA based assay and Flow Cytometry for screening and characterization. Molecular cloning and basic sequencing analysis using IMGT and BLAST.
  • Unity Biotechnology- Contractor Supplied By Pgc Workforce Solution
    Research Associate Ii. In Vivo Pharmachology
    Unity Biotechnology- Contractor Supplied By Pgc Workforce Solution Nov 2017 - Aug 2018
    Examining the efficacy of senolytic drugs in aging mice. Associated skills and techniques: IP and OG dosing. Glucose tolerance test (GTT), Insulin tolerance test (ITT), serum collection, IHC, fluorescent and confocal imaging, histology, PCR, qRT-PCR, microCT, frailty index, stem cells quantification and analysis using image J.
  • Celprogen, Inc
    Research Associate Ii
    Celprogen, Inc Dec 2016 - Jul 2017
    San Pedro, Ca, Us
    Mammalian Stem cells growing and expansion. Conventional monolayer cell culture including a 3D scaffold. Patient-derived xenograft (PDX) models of breast cancer.Associated Skills and Techniques: Mammalian cell culture, development and manufacture cell culture media. Tumor transplantation into immunocompromised mice, Fluorescent imaging, total RNA, qPCR, SOPs review and update, restocking supplies, and mouse colony management.
  • University Of Southern California
    Research Laboratory Technician Ii. Department Of Physiology And Biophysics
    University Of Southern California 2015 - 2016
    Los Angeles, Ca, Us
    Assist and performed an independent research project on the identification of Nephron Progenitor Cells and its contribution to target cell population. Associated Skills and Techniques: PCR, genotyping, tamoxifen treatment, OG dosing, C-section, mouse colony management, surgical Kidney exposure for in vivo imaging, histology including cryosectioning, immunofluorescence and imaging. Record organization, restocking laboratory supplies, ordering and preparing buffers and reagents.
  • University Of Southern California
    Research Technician Ii. Department Of Stem Cell And Medicine
    University Of Southern California Nov 2012 - Nov 2015
    Los Angeles, Ca, Us
    Assisted in two different projects which consist of removing apoptotic genes to assess kidney phenotype rescue of a mutant mice. Collaboration with the validation and identification of proximal tubules segments of mutant mice. My skills include the following techniques:• Mice colony management, setting up breeding cages, plug check, generation of double and triple KO mice through interbreeding.• Genotyping genetically modified mice by PCR technique. • Urogenital system dissection at different developmental stages.• Identification and Characterization of renal tubules markers. Lac Z staining, Cryosectioning, slide mounting and imaging• Ordering, restocking common laboratory supplies, prepare buffers and reagents.• Organizing, maintaining and updating Strain Identification Sheet
  • House Research Institute
    Research Assistant Ii. Department Of Hereditary Disorders
    House Research Institute 2011 - 2012
    Participated in noise-induce and age-related hearing loss projects. Associated Skills and Techniques: Measure hearing-evoked potentials in mice, hearing loss threshold analysis, inner ear decalcification, histology, IHC, WISH, confocal Imaging, mapping hair cells loss in the Organ of Corti via Image J, RNA sample collection and microarray, assisting analyzing data using R statistical software, and mouse colony management.
  • House Research Institute
    Research Lab Technician . Department Of Inner Ear Development
    House Research Institute Jul 2003 - Jul 2011
    I studied the role of signaling proteins in patterning the vertebrate inner ear. My skills include the following techniques:• Microinjection of mRNA and morpholinos into Xenopus laevis embryos. • Molecular biology including maxi-prep, mini-prep, restriction digests, DNA and mRNA synthesis.• Advanced embryological techniques including immunohistochemistry, in situ hybridization.• Confocal Imaging using both Leica SP5 and Zeiss 710 microscopes. • Imaging and statistical analysis of experimental data.• Preparation of reagents, buffers, ordering and restocking supplies.
  • House Research Institute.
    Certified Laboratory Animal Technician. Biomedical Research Vivarium
    House Research Institute. 2000 - 2003
    Provide high standards services of animal care in accordance and compliance with guidelines of Laboratory Animals. Associated Skills and Techniques: Monitor and care of laboratory animals, Pre and post-surgery care. Monitor for infectious diseases such as ping worms or mites, water quality for zebrafish and amphibians, treatment records, Artemia and Rotifers culture.

Aldo Castillo Skills

Polymerase Chain Reaction Dna Extraction Rna Isolation Plasmid And Genomic Dna Isolation Maxi And Mini Prep Confocal Microscopy Fluorescence Microscopy Cryosectioning Transgenic Mice Microinjection Microsoft Excel Microdissection Whole Mount In Situ Hybridization Evoked Potentials Immunohistochemistry Restriction Digestion Dna And Mrna Synthesis Data Analysis Molecular Cloning Molecular Biology Cell Culture Biochemistry Life Sciences Biotechnology Pcr Cell Biology Research In Situ Hybridization Immunofluorescence Agarose Gel Electrophoresis Microsoft Word Powerpoint Research Projects

Aldo Castillo Education Details

  • Universidad Nacional De Trujillo
    Universidad Nacional De Trujillo
    General
  • Harvard Medical School
    Harvard Medical School
    Immune Oncology
  • Uc Berkeley Extension
    Uc Berkeley Extension
    Immunology
  • Pasadena City College
    Pasadena City College
    Biotechnology

Frequently Asked Questions about Aldo Castillo

What company does Aldo Castillo work for?

Aldo Castillo works for Gilead Sciences

What is Aldo Castillo's role at the current company?

Aldo Castillo's current role is Associate Scientist.

What is Aldo Castillo's email address?

Aldo Castillo's email address is al****@****usc.edu

What schools did Aldo Castillo attend?

Aldo Castillo attended Universidad Nacional De Trujillo, Harvard Medical School, Uc Berkeley Extension, Pasadena City College.

What skills is Aldo Castillo known for?

Aldo Castillo has skills like Polymerase Chain Reaction, Dna Extraction, Rna Isolation, Plasmid And Genomic Dna Isolation, Maxi And Mini Prep, Confocal Microscopy, Fluorescence Microscopy, Cryosectioning, Transgenic Mice, Microinjection, Microsoft Excel, Microdissection.

Who are Aldo Castillo's colleagues?

Aldo Castillo's colleagues are Véronique Emonet, Hosana Ribeiro, Erin Lum, Sunil Kumar Dubyala, Borja Menendez Diaz, Chikwelu Ozigbo, Mba, Ms, Cfa, Mihaela Esanu.

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