Armelle Le Guelte Email & Phone Number

Nashville, Tennessee, United States

Nashville, Tennessee, United States

Nashville Metropolitan Area

• Designed and executed research projects deciphering the mechanism dynamic of mammary cap cells and luminal cells in the mammary gland, a key process in breast cancer development• Prepared manuscripts and grant applications• Other responsibilities include purchasing lab supplies, safety documentation, assisting, training and mentoring graduate, undergraduate and summer students

• Focused on designing and executing research projects untangling the cap or stem cell fate in mammary glands by generating and analyzing transgenic lineage tracing in vivo. I was able to demonstrate that there are no resident stem cells in murine mammary glands after birth and maturation is orchestrated by differentiated cells. However, these cells are found and proliferate in breast cancer. • Reviewed papers for different scientific journals, as well as participated on a board awarding grants.• Presented my research at national and international conferences.The murine mammary gland is a fascinating and powerful model for the study of adult stem cells. Complex morphogenetic changes occur in the mammary epithelium during puberty, pregnancy, lactation and involution. Remarkably, one mammary stem cell is able to reconstitute an entire mammary gland, but the identity of the stem cell has remained uncertain because of the absence of validated stem cell markers. A major unanswered question is whether mammary stem cells generate diverse progeny by asymmetric cell division, or through stochastic population asymmetry. It is also not known whether the polarity proteins identified in Drosophila stem cells play similar roles in mammalian stem cells. I propose to solve these important, long-standing questions, using a combination of in vivo and in vitro approaches.

Nashville, Tennessee, United States

9-month intense data science training preceded by a statistical refresher (“Statistics for Data Science”). The program focuses on practical application of Python, R and SQL to address real-world business problems by organizing and cleaning data, exploratory data analysis, machine learning, preparing data visualizations and effective data communication.Projects included:• Provider-Communities-NashvilleUsed Python (eg. pandas, etc) and PostgreSQL to prep and clean DocGraph HopTeaming (Medicare) data set to join with other data sets (eg. NPPES) containing provider and other relevant information. Finally, looked for “communities” of providers by using Neo4j (Louvain community detection algorithm) and R (ggplotly, shapefiles). The analysis revealed that providers-hospital communities are dominated by three major hospital systems and fall into geographic clusters. https://sites.google.com/view/nashvilleprovidersds4/provider-communities• Tennessee’s Opioid CrisisUsed Python (eg. SQLAlchemy, pandas, plotly) to prep and clean the Medicare Part D prescriber file. The analysis revealed a disproportionate amount of opioids prescriptions in Scott County, TN. Interestingly, this has been traced back in our analysis to just one specific prescriber, who had two time more claims per Medicare enrollee than average, and prescribed popular opioids, such as Oxycodone and Hydrocodone three to four times more than other prescribers.• Mid-Course Capstone – Extinction Is ForeverCreated an interactive R Shiny catalog of endangered species featuring different details about the species, as its habitat (geographical region), different maps as well as analyzing the potential cause for the species disappearance. https://amylg.shinyapps.io/midcourse_extinction/

Institute Cochin, Paris, France

Graduate work at a biomedical research institute. Thesis title: VE-cadherin plasticity in brain endothelial cells.I discovered that cancer stem cells (CSC) have the ability to increase blood vessels permeability in the brain: a hallmark of brain cancer progression. I determined the mode of action of CSC, opening a door to future treatment.• Teaching and mentoring experience gained through supervision of undergraduate students, ability to work with team members and collaborators• Oral presentation at a national meeting, funded a competitive Ph.D fellowshipScientific abstract:The blood brain barrier regulates the transport of molecules and cells from blood into the central nervous system. The cerebral endothelium has developed a particular system of specialized cell junctions, which carefully control the passage of plasma molecules. Vascular endothelial (VE)-cadherin is an adhesion molecule that is crucial in the organization of endothelial junctions and the maintenance of vascular integrity.Glioblastoma is the most aggressive and the most lethal brain tumor, which is characterized by a highly leaky vasculature. Furthermore, these tumors contain a subpopulation of glioma stem cells (GSC), which derive from a fraction of transformed stem cells. GSCs play a role in the tumor progression and resistance to conventional therapies. However, the involvement of GSCs in the plasticity of cerebral endothelial cells has not been studied. We have demonstrated that GSCs secrete semaphorin 3A (S3A) increased phosphorylation and internalization of VE-cadherin in cerebral endothelial cells, leading to a loss of barrier integrity. At the molecular level, we demonstrated that PP2A interacts with VE-cadherin at the basal level. Thus, PP2A stabilizes VE-cadherin, and we further showed that this complex could be disrupted by S3A produced by GSCs.

Doctor Of Philosophy (Ph.D.), Cell/Cellular And Molecular Biology

Master'S Degree, Structure, Proteome And Functional Genomics, Major In Pathological And Therapeutic Macromolecules

Bachelor Of Science - Bs, Biology, Biochemistry And Bioinformatics