Alexandra Barr abarr1@bidmc.harvard.edu Laboratory skillsCell culture, bacterial cell culture, mammalian cell culture, human tissue culture, harvesting and culture of human blood outgrowth endothelial cells (BOEC), SDS page western blot, cell lysis, GFP-trap on cell lysate, immunoprecipitation, mini prep, maxi prep, sandwich ELISA, BCA assay, nano drop, PCR, E.coli blue/white selection (IPTG Xgal selection), lentiviral transformation, siRNA transfection/ siRNA knock down, CRISPR, designing primers, thrombin stimulation and factor Xa generation assay, In-Fusion HD cloning Kit, PCR gel and DNA purification, fixing cell culture microscope slides, mixing reagents, bacterial media preparation including liquid media and petri dishes, gram staining, bacterial antibiotic resistance and production assays, bacterial swarming assay, thin layer, column, and gas chromatography, distillation, and liquid extraction, gel electrophoresis, mass spectrometry liquid chromatography data anlaysis, solvent based compound extraction column under fume hood, cryopreservation of plant and animal tissue samples in liquid nitrogen, fly crossesComputer skillsWix website builder, Inkscape vector graphics editor, ArcGIS mapmaking software, Microsoft Office applications including advanced techniques in Microsoft Excel, Google Maps My Maps editor, Joomla website design software, Blender 3D animation software, some Java, some Python, NIH BLAST, Slack, Microsoft TeamsBotany skillsPhenology data collection, plant biomass measurements, plant identification and familiarity with plant key terminology, seed germination, growing plants in greenhouse, growing plants in temperature and humidity controlled growth chamber, collecting tissue samples at proper developmental stages, determining ripening stages of rare exotic fruits, manual pollination/ plant crosses, seed saving/ cold room storage and cataloging Laboratory equipmentMicroscope, centrifuge, fume hood, sterile tissue culture hood, hot plate, TDR soil moisture measurement device, pH meter, liquid nitrogen, sonicator, tissuelyser, nano drop, UV box, autoclave, flow cytometry, mass spectrometer, plate reader, Bunsen burner, growth chamber, cell incubator