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Research Scientist with over 10 years experience in Mammalian Stem Cell Biology, including over two years experience in a Contract Development and Manufacturing Organisation (CDMO).Keen interest in human pluripotent stem cells (PSCs) and their promising cell therapy applications. Extensive experience in gene editing of PSCs - generating both knockout, gene corrected, and knock-in cell lines.Passion for scale-up of PSC and induced pluripotent stem cell (iSPC) expansion from 2D to 3D process in stirred tank bioreactors, and the subsequent differentiation to therapeutic cell types.
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Lead Scientist IiiCcrm Jan 2022 - PresentToronto, Ontario, CanadaKey experience> Project lead in Advance business unit of CCRM.> Leadership role on multiple iPSC differentiation projects, scaling up from 2D to 3D process in stirred tank Bioreactors.> Responsible for experiment planning and team coordination, as well as customer communication. -
Development Scientist IiCcrm Jun 2021 - Jan 2022Toronto, Ontario, CanadaKey experience> Project lead in Advance business unit of CCRM.> Technical lead on multiple iPSC differentiation projects, scaling up from 2D to 3D process in stirred tank Bioreactors.
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Development Scientist ICcrm Sep 2019 - Jun 2021Toronto, Ontario, CanadaKey experience> Project lead in Advance business unit of CCRM.> Culture and characterization of human induced pluripotent stem cells (iPSCs). > Technical lead on multiple gene editing projects generating knockout, gene corrected and knock-in iPS cell lines.> Collaborated with team to develop high-throughput imaging assay suitable for GMP release testing.
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Research FellowThe Hospital For Sick Children Mar 2016 - Sep 2019Toronto, Canada AreaLaboratory of Janet Rossant• Investigating the totipotent state and the progressive restriction of cell fate during early mouse embryonic development.• Analysed single-cell RNA-seq data to identify candidate transcription factors that may promote reprogramming of pluripotent stem cells (PSCs) with the ultimate goal of maintaining a totipotent stem cell in vitro.• Used CRISPR/Cas9 genome editing to generate multiple reporter PSC lines that enable isolation and characterisation of rare populations.• Performed high-throughput screening to identify pathways that activate a specific reporter.• Developed a flow cytometry assay to detect fully reprogrammed cells during forced differentiation of PSCs to the trophoblast lineage.• Assessed lineage contribution in mouse embryos by confocal microscopy. -
Postdoctoral Research ScientistThe Babraham Institute Oct 2014 - Jan 2016Cambridge, United KingdomLaboratory of Myriam Hemberger• Deciphering the Mechanisms of Developmental Disorders (DMDD) consortium member investigating a cohort of genes that display embryonic lethality, for which placental defects have also been observed.• Pioneered the use of the CRISPR/Cas9 within the group, using trophoblast stem cells (TSCs) as a model system, to investigate the extraembryonic function of this gene cohort.• Developed a high-throughput approach, utilising flow cytometry, to streamline both clone isolation and mutant screening to successfully generate a number of knockout cell lines.• Characterised mutant TSCs by trophoblast differentiation protocols. -
Phd StudentThe Babraham Institute Oct 2010 - Sep 2014Cambridge, England, United KingdomLaboratory of Myriam Hemberger• Explored models of cell fate reprogramming between embryonic and extra-embryonic lineages, and the epigenetic mechanisms that are crucial in ensuring stable cell identity.• Assessed trophoblast phenotype by observing morphological changes during reprogramming.• Characterised degree of reprogramming by transcriptome and methylome analysis.• Identified a key subset of genes that remain refractory to reprogramming and retain an epigenetic memory of their ESC origin.
Alex Murray Skills
Alex Murray Education Details
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Molecular Biology -
First Class
Frequently Asked Questions about Alex Murray
What company does Alex Murray work for?
Alex Murray works for Ccrm
What is Alex Murray's role at the current company?
Alex Murray's current role is Lead Scientist III at CCRM.
What is Alex Murray's email address?
Alex Murray's email address is am****@****ccrm.ca
What is Alex Murray's direct phone number?
Alex Murray's direct phone number is +143734*****
What schools did Alex Murray attend?
Alex Murray attended University Of Cambridge, University Of Bristol.
What skills is Alex Murray known for?
Alex Murray has skills like Pcr, Protein Expression, Cell Culture, Transfection, Stem Cells, Tissue Culture, Gel Electrophoresis, Dna Sequencing, Confocal Microscopy.
Who are Alex Murray's colleagues?
Alex Murray's colleagues are Emilia Karanxha, Amrita Verma, Aleksandra Dabkowska, Kritika A., Priya Duggal, Yi-Kai Su, Ksenia Bezverbnaya, Phd.
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