Alexander Brean Email & Phone Number

Birmingham

Birmingham, England, United Kingdom

- Planning and running large projects- Independently running R&D experiments- Communicating findings to senior leadership- Coordinating manufacturing departments- Continuous Improvement

Birmingham, United Kingdom

- Temperature driven continuous hydrophobic interaction chromatography (HIC) in a single column- Thorough chromatography method development- Research to be published in Journal of Chromatography A- SEC, HIC, FPLC, SDS PAGE, CLSM, DSC

Birmingham

- Routine troubleshooting of issues in manufacturing- Collaborated with manufacturing departments to put permanent solutions in place- Continuous Improvement- Antibody precipitation, curve activity, protein stability, antigen excess, raw material issues, antibody specificity

Chipping Campden

- Microscopic analysis of contaminants in samples- Knowledgeable of microscopic techniques- Writing reports for clients

Birmingham, United Kingdom

Project 1: Under the supervision of Professor Grant Stewart's Laboratory, I explored the role of DONSON in the DNA damage response and cell cycle regulation. Utilising techniques such as immunoprecipitation, Western blot analysis, fluorescence activated cell sorting, fluorescent microscopy, and human cell culture, I identified proteins interacting with DONSON. My contributions to this project have led to a publication in Nature Genetics.Project 2: In Dr. Aga Gambus's Laboratory, I investigated MCM7 polyubiquitylation in DNA replication fork termination using Xenopus Laevis as a model organism. This project enabled me to learn vital techniques, including chromatin isolation, working with X. laevis egg extract, bacterial cloning, PCR site-directed mutagenesis, and protein purification. Through this research, I gained a deeper understanding of the effects of blocking MCM7 polyubiquitylation on DNA replication fork termination.

Plymouth University, Davy Building, Second Floor.

At the University of Plymouth I characterised the unique properties of mouse alveolar macrophages when exposed to IL-4 and IL-13. My responsibilities included culturing alveolar macrophages, preparing whole cell lysates, and performing 2D SDS-PAGE separation, which facilitated the subsequent mass spectrometry analysis. This experience not only honed my skills in advanced laboratory techniques but also provided valuable insights into the intricacies of cellular research and collaboration in a scientific setting.

Doctor Of Philosophy - Phd, Biochemical Engineering

Developing a new thermoresponsive chromatography technique for continuous capture and polishing of proteins, monoclonal antibodies.

Mres Molecular And Cellular Biology, Life Sciences, Pass (Minor Corrections)

At the University of Birmingham, I undertook two projects involving DNA repair and DNA replication. My work completed at Professor Grant.

Bsc (Hons) Human Biosciences, Life Sciences And Human Biology, 2:1

Activities and Societies: Badminton society, student representative for 2 years.

Extended Science, Science, Distinction

Activities and Societies: Badminton society.The BSc (Hons) Human Bioscience degree offered at the University of Plymouth is primarily.