Alexander Bunk Email & Phone Number

Salt Lake City, Utah, United States

Performs FTIR spectroscopic analyses of macroscopic calculi for patient diagnostics

Developing and managing projects; designing and running experiments; creating and optimizing lab protocols; data analysis and interpretation; literature search; writing and contributing to manuscripts and grant applications; presenting work at conferences and program seminars; discussing own and colleagues' work at weekly lab meetings; participating in or leading weekly lab journal clubs; maintain and care for several different lines of wild-type and transgenic mice.Actively contributes to and helps coordinate collaborations between our lab and other labs within and outside of the University of Cincinnati.Techniques: human DRG neuron recovery and primary culture, human keratinocyte primary culture, mouse DRG neuron recovery and primary culture, mouse trigeminal ganglia neuron recovery and primary culture, human induced pluripotent stem cell-derived neuron culture, coculture of sensory neurons and keratinocytes, calcium imaging (Fura 2-AM, GCaMP6s), cAMP imaging, patch-clamp electrophysiology, qRT-PCR, immunohistochemistry, immunocytochemistry, ELISA, H&E stain, FACS, confocal and widefield fluorescence microscopy, live fluorescence and light microscopy, extensive mouse handling, routine lab maintenance, buffer and reagent preparation, statistical analysis.

Responsibilities varied, depending on the lab, but I was always responsible for learning new techniques and applying them to run experiments related to a given project, as well as performing data analysis and interpretation and routine laboratory maintenance. During my first rotation, I was tasked with and was successful in implementing a western blot protocol for general lab use.Techniques: western blot, Nanodrop nucleic acid quantitation, qRT-PCR, RNA purification, plasmid purification, mouse behavioral test scoring, H&E stain quantification, immunohistochemistry, immunocytochemistry, widefield fluorescence microscopy, cryostat sectioning, dorsal root ganglia neuron recovery and primary culture, cAMP imaging, human embryonic stem cell culture, super-resolution microscopy, FRET, routine lab maintenance, buffer and reagent preparation, statistical analysis.

Cincinnati, Oh

While in the Gross lab at CCHMC, I worked on a project studying the effects of microRNA antagonists on the expression of the A-type potassium channel subunit, Kv4.2, as well as different facets of electroencephalographic (EEG) readouts, seizure susceptibility, neuronal degeneration in the hippocampus, and dentate gyrus (DG) neuronal migration in different mouse models of temporal lobe epilepsy.Additionally, I worked on a project aimed at elucidating the effects of altered Kv4.2 expression on the expression of its associated subunits, as well as its effects on EEG characteristics.Skills which I have used and learned during my time in the lab include PCR, tissue lysis, DNA and protein gel making, western blotting, buffer making, mouse genotyping, basic mouse handling, cryostat section of mouse brain tissue, histology on mouse brain tissue (Fluoro-Jade B, Nissl staining, fluorescence immunohistochemistry), widefield brightfield and fluorescence microscopy, confocal fluorescence microscopy, quantification of stained mouse brain tissue using ImageJ or NIS-Elements software, EEG analysis using NeuroScore software, researching and optimizing procedures, and routine laboratory maintenance.

Cincinnati, Oh

I worked on DNA sequencing of tissue samples of northern mockingbirds (Mimus polyglottos) from different parts of the North American continent in order to determine if the species had diverged into different subspecies. The overall DNA sequencing procedure involved making DNA gels, PCR, gel electrophoresis, and the use of a DNA sequencer. In addition to bench work, I was expected to be able to analyze and interpret data.

Bachelor Of Science - Bs, Cell & Molecular Biology

Bachelor Of Science - Bs, Chemistry