Alexander Amerik Email & Phone Number

OBJECTIVE: Seeking a creative and challenging position (Senior/Principal Scientist, Genetics/Molecular Biology/Cell Biology/ Biochemistry) that will allow me to contribute significantly to a biotech/pharmaceutical industry. SKILL SUMMARY: Extensive experience in molecular and cellular biology, biochemistry and genetics with an especially strong background in physiology of the yeast Saccharomyces cerevisiae. Specifically, this includes metabolic engineering and design, performance and analysis of microbial fermentation experiments, mRNA purification, cDNA and genomic library construction, cloning, sequencing and PCR amplification, Northern and Southern blot analysis, expression of recombinant and engineered proteins in prokaryotic and eukaryotic systems, protein purification and characterization (enzyme kinetics, site-directed mutagenesis, Western blot analysis, pulse-chase analysis of protein degradation), protein-protein interaction (two-hybrid analysis, coimmunoprecipitation, GST pull-down assay), protein localization (indirect immunofluorescence, differential centrifugation, analysis of membrane protein trafficking and protein sorting), various genetic manipulations (gene knocking-out, mutant strain construction, chemical and genetic mutagenesis) and protein microarray-based high-throughput screening.Proficiency in the nucleic acid and protein analysis software as well as in various databases, expertise in designing research projects, competence in writing scientific papers and grant proposals. Supervisory experience includes managing research projects as a Principal Investigator and training of post-doctoral fellows, research associates and graduate students.Specialties: An expert in the field of the ubiquitin-dependent protein degradation and physiology of the yeast Saccharomyces cerevisiae.

Listed skills include Biochemistry, Pcr, Protein Purification, Protein Chemistry, and 20 others.