Alexandre Pfister Email & Phone Number

Lausanne, Vaud, Switzerland

Lausanne, Vaud, Suisse

- Manage 5+ clinical research personnel on-site (Lausanne University Hospital – CHUV) in conducting prospective Class IIa blood pressure-related medical device clinical trials study.- Coordinate with the Sponsor’s Clinical Trial Manager, Principal Investigator (PI) and other partners in implementing study procedures, timeline, inclusion/exclusion criteria, feasibility assessment, and other issues/requirement from Sponsor. - Act as a quality controller in collaboration with the CRO during monitoring visits, resulting to a timely and accurate collection of the clinical study data.- Perform a data cleaning, monitoring and management for further statistical analyses.- Monitor the adherence to clinical trials protocols and alignment with internal SOPs, ICH guidelines, Good Clinical Practices, ISO standards and Swiss Law.

Lausanne, Vaud, Switzerland

- Participating in various clinical research studies through different projects taking place in the nephrology and hypertension service of CHUV.- Knowledge of the Good Clinical Practice (GCP).- Recruiting participants with electronic patient records (Soarian program).- Collecting data from participants and managing Case Report Form (CRF).- Design, development and review of study protocol, Case Report Form (CRF) and Informed Consent.- Project submission to the Swiss Ethical Committee.- Statistical data analysis using the Stata program.

Monthey, Valais, Suisse

- Developing an in vitro diagnostic Multiplex prototype to screen bloodborne pathogens.- Acquired strong knowledge in ELISA Multiplex.- Actively participated in the laboratory biosafety guidelines development.- Skilled in the use of the sciFLEXARRAYER S3 spotter from SCIENION.

Lausanne, Suisse

Gymnase D'Yverdon

- Demonstrated excellent teaching skills through one-year pedagogic education training. - Prepared and taught lectures/practicals of biology to students between 15 and 18 years old.- Evaluated performance, gave constructive feedback, and motivated students. - Explained complex or difficult concepts in basic terms and language.

Changins, Mycology And Biotechnology Group

Project title: Setting up a qPCR detection system in order to quantify the major vineyard fungal pathogens from air samples:Skills:- Set up and optimized a qPCR protocol for absolute quantification of vineyard fungal pathogens using spores concentration in the air. This protocol can now be routinely used in the laboratory in order to answer various epidemiological questions.- Designed qPCR primers and realized standard curves.- Performed, optimized and analyzed qPCR reactions that allowed the absolute quantification of spores in the air.This project was done under the supervision of Dr. Pierre-Henri Dubuis.

Lausanne

Lausanne, Suisse

I planned and conducted a research project in the group of Pr. Niko Geldner on Casparian strip (CS), a filter localized in the root endodermis of plants and expected to be crucial for water and ions movement through the root.• Skills:- Strong scientific knowledge in plant biology and physiology, molecular and cell biology- Planned, organized and conducted autonomously a research project from the beginning to the end, leading to a publication as first author in a peer reviewed journal - Cooperated with all team members, established and coordinated successful collaborations with national and international research groups (Switzerland, France, Japan, Scotland and Germany)- Wrote, reviewed and published scientific reports at all levels — from brief abstract to book-length manuscript — in French and English- Presented complex scientific findings in a straightforward way through oral presentations in lab meetings, department meetings and national/international conferences -Taught lectures/practicals to Bachelor students and supervised junior lab members- Planned, developed and validated numerous molecular biology protocols (PCR, RT-PCR, qPCR, all steps of cloning, yeast two-hybrid system, Western-Blot, genotyping)- Operated routinely fluorescence and confocal microscopes: fluorescent proteins localization, Fluorescence Recovery After Photobleaching (FRAP), 3D reconstruction, time-lapse realization, 2Photon microscopy- Responsible of the management and ordering of chemical products for the department• Strong expertise of several laboratory technics:- PCR, cloning, sequencing, qPCR, reporter genes, Western-Blot, protein-protein interactions- Bacteria and yeast handling/transformation, yeast two-hybrid screen, split-ubiquitin- Extensive knowledge of microscopy (fluorescence, confocal, 2Photon), live imaging, FRAP, proteins localization, time-lapses- Plants: Transformation, crossing, hydroponic, physiological experiments

Région De Lausanne, Suisse

• Participated in teaching program for Bachelor students by optimizing the experimental set up, introducing the topics by oral presentations, guiding the students during their experiments as well as by evaluating written reports.- Lab course in Plant Cell Biology for 1st year Bachelor students in 2010, 2011, 2012 and 2014 (144 hours of teaching)- Lab course in Plant Physiology and Development for 2nd year Bachelor students in 2010 (56 hours of teaching)- Lab course in Live imaging for 3rd year Bachelor students in 2010, 2011 and 2012 (24 hours of teaching)- Lab course in Molecular Biology for 3rd year Bachelor students in 2011 and 2012 (120 hours of teaching)• Participated three times in “Les mystères de l’UNIL", open days at the University of Lausanne aiming to show lab research to a public audience. - Involved in the organization and the animation of the event- Communicated scientific research to a broad audience

Région De Lausanne, Suisse

During my master project, I was interested in understanding the factors involved in plasma membrane localization of CASP1, a protein involved in Casparian strip formation in plant root endodermis.Skills:- Molecular cloning- Confocal microscopy- Protein-protein interaction using the split-ubiquitin system- Western blot- Scientific writing and presentation of results

Région De Lausanne, Suisse

About 70% of plants, including many crops such as wheat and rice, enter a symbiotic mycorrhizal association with a fungus. During this association, plant roots gives to the fungus sugars and get in return water and mineral nutrients. In order to initiate this symbiosis, both organisms enter into communication and need to recognize their partner in order to avoid any defense response.During my premaster project, I was investigating rice proteins that might have the ability to detect fungal cell walls. To do so, the proteins of interest were down-regulated by RNA interference (RNAi) and my first goal was to check the effectiveness of this down-regulation by diverse molecular techniques. The second aim was to observe a potential effect on root mycorrhiza capacity of these rice mutant plants by mycorrhizae staining and quantification.This project was done under the supervision of Dr. Uta Paszkowski, Department of Plant MolecularBiology (DBMV), Lausanne, Switzerland.Skills:- Southern blot- RNAi- RT-PCR- RNA extraction- Rice cultivation- Mycorrhizae staining and quantification

Phd In Life Science, Cellular And Molecular Biology

Dissertation entitled "Role of an LRR Receptor-Like Kinase in the establishment of a functional root endodermal barrier" Under.

Master Of Science (Msc), Genomic And Experimental Biology

Bachelor'S Degree, Biology