Alex Yi-Lin Tsai Email & Phone Number

美國 麻塞諸塞州 波士頓

美國 麻塞諸塞州 波士頓

台灣 Taiwan 新竹市

I was responsible for metabolic engineering of microbes to improve the production of dyes for textile dyeing applications.

South San Francisco

I am responsible for the development of molecular and genetic tools in non-model organism to improve the strain engineering capabilities of the methane bioconversion program. In addition to bench work, I have added leadership responsibility by managing research assistants. To ensure the success of the program, I coordinate and collaborate with members from.

San Francisco, CA

• Methane bioconversion program for the production of fuels and value-added compounds
• HTP DNA construction via yeast assembly
• Characterization of key enzymes in the methane bioconversion pathway via microbial engineering
• Development of HTP screen to characterize growth phenotype of engineered microbes

Emeryville, CA

• Investigate the role of plant acyl- and glycosyl transferases in substitution of xylan backbone during plant cell wall biosynthesis.
• Recombinant expression of plant acyl- and glycosyl transferases (cytosolic and membrane proteins) in E. coli, S. cerevisiae, and N. benthamiana
• Purification of the plant enzymes using affinity, ion exchange, and size exclusion chromatography with FPLC (ex. GE ÄKTA)
• Functional characterization of plant transferases by identifying the catalytic activity and screen for substrate specificity with in vitro activity assay or engineered yeast heterologous expression system
• Experienced with HPLC, LC/MS, UV/Vis spectroscopy, and MALDI as means to detect products from the enzyme reactions

Toronto, Canada Area

• Transgenic expression of microbial cell wall modifying enzymes in planta as a mean of cell wall engineering
• Construct library of transgenic Arabidopsis thaliana expressing microbial carbohydrate active enzyme with 20+ enzyme activities
• Analyses of transgenic plant fibres at the morphological, cellular (microscopy, histological staining, immunolocalisation), and molecular (FT-IR, composition analyses, saccharification, xylan extractability) levels
• Cloning, expression (ex. E. coli and P. pastoris), purification (ex. affinity chromotography), and characterisation (ex. developed assay to measure substrate specificity, reaction optima, temperature/pH stability, and.
• Transcriptome profiling of the transgenic Arabidopsis using RNA-seq
• Developed analytical platform of differentiating Arabidopsis tissues and mutants based on cell wall chemistries using time-of-flight secondary ion mass spectrometry

Toronto, Canada Area

• Courses: Molecular Biology (BIO240), Cell and Developmental Biology (BIO241), Eukaryote Gene Expression (CSB349)
• Present lecture materials and lead tutorial discussion
• Supervise students through molecular and cell biology labs
• Coordinate students through group course project
• Preparation and grading of teaching materials, quizzes, assignments and term tests

Yilan County, Taiwan

• Cloning and characterisation of a macrophage marker from orange-spotted grouper

Toronto, Canada Area

• Cloning, expression, and purification of a bacteriophage tail protein
• Characterisation of the iron-sulfur clustering binding of the bacteriophage protein using UV absorbance and site-directed mutagenesis

Toronto, Canada Area

• Ab initio modelling and characterisation of beta-lactam antibiotic structures

Toronto, Canada Area

• Performing phylogenetic footprinting analyses for various members of Brassicae family: including sample preparation and bioinformatics

Doctor Of Philosophy (Ph.D.), Cell And Systems Biology

B.Sc. (Hon.), Biochemistry