Alice Wiedeman Email & Phone Number

Seattle, WA

• Facilitated scientists of diverse training and expertise to produce the best data from precious human samples to enable discoveries about the phenotype and function of immune cells in human diseases. Managed the.
• Oversaw the operations of the core including vision/direction, budgeting/billing, establishing research projects with internal and external clients, and the supervision and training of four technicians.
• Supported team science investigating mechanism(s) of action and immunophenotypic indicators of outcome and therapeutic response in human immune diseases in partnership with other scientists, clinicians, non-profit.
• Managed the mass cytometry (CyTOF) services, including coordination with internal and external clients, training, and routine maintenance of the CyTOF instrument.
• Expanded the cytometric capabilities of the core by incorporating antigen-specific cell identification, functional assays (cytokine, activation, proliferation, signaling/phosphorylation), and sample barcoding, as well.
• Utilized cutting-edge cytometric technology and collaborated with the Bioinformatics group to incorporate and develop customized high-parameter data analysis tools.

Everett, WA

• Developed cell-based, flow cytometric, and ligand-binding assays in a GLP environment in support of non-human primate and rodent preclinical toxicology studies.
• Managed the flow cytometry and cell-based assay services, including supervision of two direct reports and routine maintenance of the instruments.
• Worked with clients to develop and validate flow cytometry panels, cell-based assays, and immunoassays (ELISA and Luminex) under Good Laboratory Practices (GLP) for use in regulated preclinical studies of non-human.
• Provided contributing reports for flow cytometry and immunoassays, wrote and improved SOPs, and served as a scientific resource for junior staff, peers, and study directors.

Seattle, WA | Lab Of Keith B. Elkon, MD, Division Of Rheumatology

• Studied adaptive immune cell development and regulation in autoimmune-prone mice. Elucidated the cellular and biochemical mechanisms regulating human plasmacytoid dendritic cell (pDC) function in health and disease.
• Utilized bioassays incorporating flow cytometry, including phos-flow and imaging flow cytometry, in addition to confocal microscopy and ELISA to elucidate the cellular and biochemical mechanisms regulating the rare.
• Performed flow cytometry and cell sorting to assess adaptive immune cell development and regulation in autoimmune-prone mice, and evaluated autoreactive B cell function by ELISPOT and in vitro cellular assays to assess.
• Initiated and maintained productive collaborations with external companies and internally with other labs to share expertise and reagents, resulting in increased funding and multiple publications.
• Prepared and presented posters for international research meetings, aided in the writing of successful grant applications, and authored peer-reviewed scientific journal articles.

Seattle, WA | Lab Of Anne M. Stevens, MD/PhD, Immunity And Immunotherapies

• Investigated the role of dysregulated immune tolerance in the pathogenesis of pediatric lupus. Studied the effects of pediatric thymectomy on peripheral blood immune cell composition and function.
• Developed novel multicolor flow cytometry panels for the evaluation of rare human leukocyte subsets and biomarkers of active autoimmune disease.
• Optimized conditions for human mixed leukocyte reactions and the flow cytometric evaluation of responder T cell cytokine production and proliferation using a 10+ parameter panel.
• Managed all day-to-day operations of the lab, and maintained and organized inventories of thousands of human samples and associated data sets.

Claremont, CA | Lab Of David J. Asai, Phd, Biology Department

• Researched the function of components of the molecular motor dynein.
• Identified two light-chain 4 (LC4) genes of the molecular motor dynein in Tetrahymena thermophila and utilized molecular cloning techniques to generate plasmids to knock out LC4.

Phd, Immunology, 3.8 Gpa

Activities and Societies: Graduate and Professional Student Senate, 2009-2010 Earned a PhD in Immunology with a Certificate in Molecular.

Certificate, Molecular Medicine

The Molecular Medicine Training Program (MMTP) at the University of Washington is a certificate program designed to train Ph.D..

Bs, Molecular Biology And Chemistry, 3.7 Gpa

Activities and Societies: Phi Lambda Upsilon (Honorary Chemical Society), 2005 Graduated with Honors in Molecular Biology and in Chemistry.