Aliya Datoo Email & Phone Number

Toronto, On, Ca

Toronto, On, Ca

Toronto, Ontario, Canada

DPLM-Genome Diagnostics: Molecular GeneticsSequencing-Illumina TruSeq PCR-free WGS library prep-Agilent SureSelectXT TargetEnrichment System for the Illumina platform-Use and maintenance of the Agilent BRAVO liquid handling platform-DNA sample QC using the Agilent Tapestation (automated electrophoresis)-KAPA library quantification qPCR (QuantStudio3)-Pooling and loading WES/WGS libraries on the Illumina Novaseq and MiSeq-Fragmentation (Covaris)MLPA-Regular and methylation sensitive (MRC Holland protocol)-Fragment separation (ABIx3730)-QC Data analysis (GeneMarker)

London

Helped to manage the laboratory processes for Genomics England's Whole Genome Sequencing project to Illumina. My role also largely involved Quality Management (audits, CAPAs, reviewing SOPs, preparation for UKAS), training, genetic analysis and molecular reporting (CF-HT4).

London, England, United Kingdom

Duties included data entry, database management, transcribing reports and I have become proficient in using medical terminology and software.

London, England, United Kingdom

-Carried out molecular genetic testing for haematological malignancies including BCR-ABL, FLT3/NPM1 and other myeloproliferative disorders (qPCR, PCR, Sanger Sequencing, NGS). -Ensured that turnaround times (TATs) were met effectively, e.g., during the beginning of the pandemic, solely carried out for the complete technical workload for the BCR-ABL service for 100% TATs.- QC analysis for qPCR, ApoE genotyping and multiplex PCRs (capillary gel electrophoresis).- Involvement with validations/verifications for service improvements (GenePrint24, BCR-ABL fragment analysis).-Contributed to writing SOPs, audits, and UKAS inspections. -Trained staff and helped manage workload for technical team.

-Carried out histology and genetic testing for amyloid. -Maintenance of Sanger sequencer, Bond Machine, ClearVue cover slipper. -Performed gel electrophoresis, PCR clean-up, DNA extraction, ethanol precipitation.-Carried out Congo Red and H&E staining, embedding, immunohistochemistry. -Observed and appreciated the use of Next-Generation-sequencing and Mass Spectrometry inclinical diagnosis of amyloid.

London, England, United Kingdom

-Successfully validated the IgE-FAB assay for HDM allergens after immunotherapy (IT).-Skillfully analysed allergen-dose responses, immune dependency, and assay variability.-Organized and led lab observations and data interpretation sessions.-Participated in additional projects (Foxp3 and antibody responses) to enhance manual dexterity.-Mammalian cell culture-Became proficient in using ELISA, q-PCR, PBMCs and T-reg isolation, microscopy and FACS.

London, England, United Kingdom

-Organizing administrative work, e.g., collating feedback from society members to improve future events.-Negotiating and securing funds from external organizations.-Maintenance of annual budget and submission of payment slips.

Master'S Degree, Genomic Medicine, Merit

Modules: Omics, Genomics of Common and Rare Diseases, Fundamentals in Human Genetics/Genomics, Bioinformatics, Pharmacogenomics, Advanced.

Cpd, Risk Management, Pass

Introduced to the main concepts of Risk Management; risk identification, quantification and control and approaches to assessing.

Bachelor Of Science (Bsc), Biomedical Sciences, General, 2Nd Class (Honours)

Activities and Societies: Jazz Society, RnB Hip Hop Society (Treasurer), VOCE Choir, stAR representative Dissertation: “The role.