Allison Fleck Email & Phone Number

Boston, Ma, Us

Waltham, Massachusetts, United States

Greater Boston Area

• Support vaccine development for Dengue, Norovirus and Zika at Takeda Vaccines• Analyze samples for potency and purity using immunofocus assays, alphaLISA, SDS-PAGE and HPLC assays • Implement and validate Hamilton liquid handling robot protocols

• Led project investigating targeted drug delivery of chirality and permeability influencing drugs to vascular cells using liposome and exosomes • Produced and characterized vesicles using ELISA and DLS • Characterized drug delivery efficiency of hydrophilic and amphipathic drugs • Used micropattern assays to determine cell chirality

PhD Candidate supervised by Professor Leo Q Wan, osteoarthritis research on the synovial joint • Extensive experience in biochemical techniques, cell culture, tissue culture, mechanical testing and MR imaging• Used MRI to map cartilage degradation and mechanical properties• Designed, fabricated, ran and analyzed a bioreactor to culture synoviocytes to create engineered synovial fluid• Cultured bovine cartilage explants to test treatments for osteoarthritis • Used transwells to induce growth factor gradients in MSC tissue engineered cartilage constructs• Prepared samples for MRI, explant culture and synovial fluid studies from bovine stifle joints• Mentored three undergraduate students, all three of whom contributed to published manuscripts; One presented a poster at the Northeastern Bioengineering Conference

Undergraduate Research Assistant, Senior Thesis in osteochondral biomineralization supervised by Professor Lara Estroff• Developed and tested a mini-double diffusion system capable of incorporating cells to explore the effects of chondrocytes on hydroxyapatite crystallization for applications in bone repair• Characterized mineral samples using x-ray diffraction• Harvested and cultured primary bovine chondrocytes for incorporation in agarose gel scaffolds in double diffusion bioreactor • Awarded first place for Senior Thesis Poster

Ithaca, New York Area

Research and Development Intern in anti-microbial coating research • Prepared and tested anti-microbial coatings for collagen scaffolds• Characterized anti-microbial efficacy through zone of inhibition and most probable numbers assays on Pseudomonas aeruginosa cultures• Characterized biocompatibility of coatings using MTT, live/dead and infiltration assays• Developed a spectrophotometric assay to determine the concentration of silver nanoparticles in solution

Boston, Ma

REU Researcher in polymer-based nanocarbon composites supervised by Professor Marilyn Minus• Studied processing of polymer/nanocarbon composite fibers to enhance toughness• Created a fiber with a similar toughness as KevlarTM at a similar elongation-to-break• Characterized samples using differential scanning calorimetry and dynamic mechanical analysis

Portland, Me

Research and Development Intern in renewable tidal energy research • Extracted and analyzed data from circulation models for current speeds at tidal turbine sites• Estimated tidal harmonics from Acoustic Doppler Current Profiler data and circulation models• Developed a MATLAB model to estimate the probability of debris impacting a tidal turbine

Doctor Of Philosophy (Ph.D.), Biomedical Engineering

Bachelor Of Science (Bs), Materials Science And Engineering, Biomedical Engineering

Activities and Societies: Big Red Marching Band, Big Red Pep Band, Cornell Steel Drum Ensemble, Phoenix Society, MUSE America, Women.