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Experienced researcher well versed in cell biology, biochemical techniques, MRI, as well as material and mechanical characterization techniques.
Flagship Pioneering
View- Website:
- flagshippioneering.com
- Employees:
- 772
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Senior ScientistFlagship PioneeringBoston, Ma, Us
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Senior ScientistResilience Feb 2021 - PresentWaltham, Massachusetts, United States -
Senior ScientistPpd Oct 2019 - Jan 2021Greater Boston Area• Support vaccine development for Dengue, Norovirus and Zika at Takeda Vaccines• Analyze samples for potency and purity using immunofocus assays, alphaLISA, SDS-PAGE and HPLC assays • Implement and validate Hamilton liquid handling robot protocols -
Postdoctoral ResearcherRensselaer Polytechnic Institute Jan 2019 - May 2019• Led project investigating targeted drug delivery of chirality and permeability influencing drugs to vascular cells using liposome and exosomes • Produced and characterized vesicles using ELISA and DLS • Characterized drug delivery efficiency of hydrophilic and amphipathic drugs • Used micropattern assays to determine cell chirality -
Graduate Research AssistantRensselaer Polytechnic Institute Jul 2013 - Dec 2018PhD Candidate supervised by Professor Leo Q Wan, osteoarthritis research on the synovial joint • Extensive experience in biochemical techniques, cell culture, tissue culture, mechanical testing and MR imaging• Used MRI to map cartilage degradation and mechanical properties• Designed, fabricated, ran and analyzed a bioreactor to culture synoviocytes to create engineered synovial fluid• Cultured bovine cartilage explants to test treatments for osteoarthritis • Used transwells to induce growth factor gradients in MSC tissue engineered cartilage constructs• Prepared samples for MRI, explant culture and synovial fluid studies from bovine stifle joints• Mentored three undergraduate students, all three of whom contributed to published manuscripts; One presented a poster at the Northeastern Bioengineering Conference
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Research AssistantEstroff Group, Cornell University Jan 2011 - Jun 2013Undergraduate Research Assistant, Senior Thesis in osteochondral biomineralization supervised by Professor Lara Estroff• Developed and tested a mini-double diffusion system capable of incorporating cells to explore the effects of chondrocytes on hydroxyapatite crystallization for applications in bone repair• Characterized mineral samples using x-ray diffraction• Harvested and cultured primary bovine chondrocytes for incorporation in agarose gel scaffolds in double diffusion bioreactor • Awarded first place for Senior Thesis Poster
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InternIfyber May 2012 - Aug 2012Ithaca, New York AreaResearch and Development Intern in anti-microbial coating research • Prepared and tested anti-microbial coatings for collagen scaffolds• Characterized anti-microbial efficacy through zone of inhibition and most probable numbers assays on Pseudomonas aeruginosa cultures• Characterized biocompatibility of coatings using MTT, live/dead and infiltration assays• Developed a spectrophotometric assay to determine the concentration of silver nanoparticles in solution -
Reu Research AssistantNortheastern University Jun 2011 - Aug 2011Boston, MaREU Researcher in polymer-based nanocarbon composites supervised by Professor Marilyn Minus• Studied processing of polymer/nanocarbon composite fibers to enhance toughness• Created a fiber with a similar toughness as KevlarTM at a similar elongation-to-break• Characterized samples using differential scanning calorimetry and dynamic mechanical analysis -
InternOcean Renewable Power Company Dec 2010 - Jan 2011Portland, MeResearch and Development Intern in renewable tidal energy research • Extracted and analyzed data from circulation models for current speeds at tidal turbine sites• Estimated tidal harmonics from Acoustic Doppler Current Profiler data and circulation models• Developed a MATLAB model to estimate the probability of debris impacting a tidal turbine
Allison Fleck Skills
Allison Fleck Education Details
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Biomedical Engineering
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Materials Science And Engineering, Biomedical Engineering
Frequently Asked Questions about Allison Fleck
What company does Allison Fleck work for?
Allison Fleck works for Flagship Pioneering
What is Allison Fleck's role at the current company?
Allison Fleck's current role is Senior Scientist.
What is Allison Fleck's email address?
Allison Fleck's email address is al****@****ppd.com
What is Allison Fleck's direct phone number?
Allison Fleck's direct phone number is +120770*****
What schools did Allison Fleck attend?
Allison Fleck attended Rensselaer Polytechnic Institute, Cornell University.
What skills is Allison Fleck known for?
Allison Fleck has skills like Reverse Transcription Polymerase Chain Reaction, Research And Development, Spss, Joint Dissection, Bioreactor, Autocad, Elisa, Cell Biology, Western Blotting, Microsoft Powerpoint, Matlab, Diffusion Tensor Imaging.
Who are Allison Fleck's colleagues?
Allison Fleck's colleagues are Jun Jie (Jj) Ong, Julia Walsh, Jasmine Quynh Le, Vinidhra Mani, Dhanasheel Muralidharan, Karl Biggs, Ali Struyk.
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Allison Raimondo
Admissions Enrollment Manager At Rocky Mountain College Of Art + DesignLittleton, Co1csuglobal.edu1 +130351XXXXX
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