• Designed and executed 3 projects in collaboration with chemical and cell biologists to study the onset and progression of centromere-kinetochore assembly during cell division and implications in chromosomes inheritance. • Designed and created the repair templates/guides for bi-allelic gene replacement, CRISPR-cas9 gene editing, tagging/knockin/knockout proteins and Flp-FRT site-specific recombination-based editing in human cells.• Developed and executed cell based assays to drug-induced crosslinking and controlled reversible dimerization of macromolecules and study protein-protein dynamics in cells.• Quantitate analysis of centromere protein complex dynamics using ImageJ/Fiji image analyses in cells• Established tools and analyzed the precise role of CENP-C mutants in centromere functions and role in chromosome segregation. • Designed and Constructed insect cell and bacterial cell expression systems of 17 kinetochore proteins.

• Designed, developed, and executed 3 collaborative projects with structural scientists, uncovering key insights into centromere function in chromosomes inheritance, published in 3 papers. These works significantly advanced our understanding of centromere stability and its implications in cancer biology.• Purified, and solved the structures of human core centromere nucleosomes complex using Cryo-EM, RELION, cryoSPARC tools.• Designed and implemented high-precision biochemical purification tools for diverse biomolecules like DNA, histones, and polymerases, ensuring a steady stream of high-quality samples essential for collaborative research and industrial applications.• Identified the novel functional compositions of CENP-C and CENP-N on CENP-A nucleosomes, this research contributes for future research aimed at combating diseases associated with centromere malfunction. • Developed invitro and cell-based assays to quantify the dynamics of biomolecules, stability of nucleo-protein complexes. • Designed and executed the generation of monoclonal cell lines through CRISPR/Cas9, transduction and FACS sorting techniques. • Executed cell survival, colony formation and localization experiments efficiently to study the functions of centromere in chromosomes biology.• Mentored/supervised a range of junior scientists, including biochemists and molecular biologists.

• Executed a large-scale microRNA knockdown screen in Drosophila for understanding mitochondrial morphology and disease mutants.• Generated and Maintained Drosophila strains, performed morphology phenotypes and Mito tracker-based assays for studying mitochondrial biogenesis.

Used biochemical and functional techniques to analyze the stability of wheat proteins.