Protein Manufacturing Associate Ii
Current• Performed bioprocessing procedures within cell culture, purification, and buffer preparation in a commercial manufacturing team in a cGMP environment • Followed standard operating procedures and documentation involving basic laboratory techniques, calculations, materials measurement, and operation of laboratory equipment • Experienced in setup of 3-liter, 7-liter, and 20-liter small scale bioreactors to the Applikon ez2-Control tower • Prepared up to 100 liters of buffer for fermentation and protein purification processes• Filtrated buffer, resuspended cell pellet, and protein elution via vacuum and peristaltic filtration• Experienced in writing methods and running the Akta Pilot 600 chromatography system to purify proteins via FPLC columns• Setup various Tangential Flow Filtration systems to isolate the protein of interest via concentration, diafiltration, and fractionation• Proficient in column packing with Cytiva HiScale and BPG columns for affinity and ion exchange chromatography • Experienced in drafting various standard operating procedures for conductivity measurements and biowaste removal and regulation • Proficient in running SDS-page gels and HPLC to determine the purity and presence of various manufactured proteins • Cross department training on processes and equipment uses• Executed successful validation protocols and reports of new protein transfers from Research and Development• Submitted Document Change Requests in GAS (Grand Avenue Software document control system) to update internal use documents
