Alyssa Holman Email & Phone Number

• Differentiation, fluorescence-based sorting, and injection of cardiac cell types from human induced pluripotent stem cells for cell therapy applications in a mouse myocardial infarction model

La Jolla, California

• Generated cardiac cell types from human pluripotent stem cells in monolayer and organoid systems using small molecules and growth factors for high-throughput CRISPR-based screens, single-cell multi-omic sequencing, and cardiac cell therapy• Designed, executed, and analyzed large-scale and targeted CRISPR-based gene editing screens with follow-up functional validation to characterize screen hits in stem cell-derived cardiac preclinical models• Performed range of functional assays including calcium and immunofluorescence imaging, microelectrode arrays, and oxygen consumption rate measurement to assess cardiac maturity on stem cell derived cardiomyocytes• Analyzed single-cell RNA-sequencing, single-nucleus ATAC-sequencing, and bulk RNA-sequencing data from hPSC-cardiomyocytes and in vivo fetal heart tissues using well-established pipelines in R and Python• Stem cell line engineering through generation of CRISPR-Cas9 knockouts and genetically encoded reporters• Designed novel libraries and incorporating literature-supported libraries for CRISPR-a and -KO screens• Collaborative research project with a group at Carnegie Mellon testing engraftment of cardiomyocytes in mouse

San Francisco Bay Area

• Established three in vitro bioengineering cell assays to mature cardiomyocytes and muscle cells • Developed a platform in Matlab to analyze calcium kinetic imaging and cellular alignment images• Introduced traction force microscopy as a potential assessment for cardiovascular candidate therapeutics

Ithaca, New York Area

• Investigated the potential of grass carp heart extracellular matrix (ECM) to regenerate heart tissue by promoting cardiomyocyte proliferation, preventing myofibroblast differentiation, and promoting angiogenesis

Stanford, Ca

• Assisted in making pure populations of vein and artery endothelial cells from human pluripotent stem cells using small molecules and growth factors; analyzed data through RT-qPCR, flow cytometry, and immunostaining

Cornell University

• Performed multiple genome wide association studies using a linear mixed model, conducted RNA-sequencing, and applied RNA-interference to analyze the impact of repeat expansions in the Drosophila model for ALS

Carlsbad, California

• Evaluated antisense oligonucleotides across diverse in vitro liver models and in vivo rat studies via RT-qPCR, ELISA, and histology to investigate drug potency variations between animal models

Stanford University

• Assisted in data collection and figure generation for a manuscript on 3-D microwell system for tumor cells• Built a device to levitate cells using magnetic microparticles and used this device to grow blood vessels

Doctor Of Philosophy - Phd, Biomedical Sciences

NIH (T32) Predoctoral Genetics Training Grant Awardee Ruth L. Kirschstein Predoctoral Individual NRSA (F31) Fellowship Awardee

Master Of Engineering - Meng, Biomedical Engineering

Bachelor Of Arts (B.A.), Biology And Society