Amanda Baumholtz, Ph.D. Email & Phone Number

Cambridge, Ma, Us

Waltham, Massachusetts, United States

• Developed and optimized antibody and peptide discovery platform from library construction to hit screening.• Built in-house antibody and peptide library generation capabilities for in vitro display platforms.• Design and optimize strategies to lift immune repertoires from immunized animals to create immune libraries.• Build strategies for selection and screening of mAbs, protein domains and peptides using phage display from immune, naïve and synthetic libraries.• Design and execute phage-display antibody discovery campaigns using synthetic, naïve, and immunized repertoires.• Perform biophysical characterization experiments (ELISA and Octet BLI) to screen hits from discovery campaigns.• NGS sample preparation and analysis.• Design and execute strategies to amplify and clone antibody variable regions, protein domains or peptides from display libraries into final therapeutic format.• Manage CROs.• Represent Biotherapeutics Engineering function at project team and group meetings.• Lead antibody discovery team.• Keeps a detailed and well-organized electronic laboratory notebook.

Montreal, Quebec, Canada

Montreal, Quebec, Canada

- Design, clone, and optimize constructs for expression of antibodies in various formats (Fc, VHH/nanobody/sdAb, scFv, multimeric) in prokaryotic and eukaryotic systems using molecular biology techniques (primer design, PCR, golden gate assembly, site-directed mutagenesis)- Design, construct and optimize antibody phage display libraries from immunized and naïve animals (mouse VH, camelid VHH, and scFv)- Design phage display panning campaigns to isolate sdAbs- Maintenance of plasmid and glycerol stock inventory- Mouse genotyping - Training and supervision of junior staff- Write Standard Operating Procedures and maintain an electronic laboratory notebook- Analyze, report, and present experimental results in both report and presentation formation at company meetings- Liaise and coordinate projects across departments and with CROs to ensure timely delivery of company goals- Represent the Molecular Biology group at project team meetings

Montreal, Quebec, Canada

Constructing fate maps of human and mouse spermatogonial stem cellsImproving the homing efficiency of spermatogonial stem cells • Presented findings at 1 international and 2 local conferences;1 best oral and 1 best poster presentation award• Teaching INDS 123 & 223: Research Fundamentals 1 and 2, Faculty of Medicine, McGill University, Montreal, QC, Canada

Montreal, Canada Area

• Wrote and submitted 2 and edited 3 manuscripts published in peer-reviewed scientific journals • 10 oral and 21 poster presentations at conferences; 4 awards for best scientific presentation• Managed multiple projects and collaborated with a multidisciplinary team of scientists and clinicians within the RI-MUHC and Europe• Supervised 6 undergraduate students and trained 4 graduate students • Responsible for overseeing laboratory purchases, including equipment and materialsProject: Characterizing Claudin-dependent morphogenetic events during neural tube closure and the impact of CLDN variants• Showed that claudins are required for normal apical localization of proteins in the neural epithelium to regulate the cell shape changes and tissue movements that regulate neural tube closure in chick and mouse embryos• Performed targeted Next Generation Sequencing and bioinformatics analysis on a cohort of patients with neural tube defects to identify potential pathogenic mutation• Showed that variants do not affect claudin localization to tight junctions in transfected cell lines but a subset caused neural tube defects when overexpressed in chick embryos Project: Facilitating engraftment of male germ line stem cells: Toward developing a novel fertility restoration strategy for boys with cancer• Performed immunofluorescence on mouse and human testes to identify claudin family members expressed in the blood-testis-barrier • Generated proof-of-concept experiments demonstrating that peptides targeting claudins transiently disrupt the tight junction paracellular barrier using size-selective permeability assaysProject: Evaluating the effect of nutraceuticals on Claudin expression and localization• Showed that treating chick embryos with quercetin affects claudin expression and causes an increase in cranial neural tube defects in chick embryos that this supplement may be harmful for pregnant women

Montreal, Canada Area

• Monitored and interacted with 25-50 clinical trial participates daily • Worked on early phase and bioequivalence clinical trials daily preparing, collecting and processing blood/urine samples in accordance with set timelines and SOPs• Received ICH-GCP and GLP certification

Postdoctoral Research Fellow, Experimental Medicine

Activities and Societies: Executive Council, Postdoctoral advisor, Experimental Medicine Graduate Student Society, McGill University.

Graduate Certificate In Business Administration, Business Administration And Management, General

Doctor Of Philosophy (Ph.D.), Human/Medical Genetics

Activities and Societies: Society for Cell Biology; American Society for Cell Biology; American Society of Human GeneticsThesis title.

Bachelor Of Science (B.Sc.), Cell Biology And Anatomy