Undergraduate Researcher
CurrentServe as undergraduate biochemistry researcher in the Jackrel Lab in the Washington University Chemistry Department.Engineer variants with mutations in nucleotide-binding domain 1 (NBD1) of protein Hsp104 to assess therapeutic potential on substrates involved in neurodegenerative diseases.Screen variants selected from deep mutational scan of NBD1 in yeast model for subsequent validation in mammalian cells.Execute primer design and QuikChanges for site-directed… Show more Serve as undergraduate biochemistry researcher in the Jackrel Lab in the Washington University Chemistry Department.Engineer variants with mutations in nucleotide-binding domain 1 (NBD1) of protein Hsp104 to assess therapeutic potential on substrates involved in neurodegenerative diseases.Screen variants selected from deep mutational scan of NBD1 in yeast model for subsequent validation in mammalian cells.Execute primer design and QuikChanges for site-directed mutagenesis; utilize Sanger sequencing to confirm that variants have successfully mutated.Perform yeast transformations, spotting assays to assess variant's specific toxicity rescue on substrates, microscopy to evaluate variant's disaggregation activity, temperature sensitivity assays, thermotolerance assays, and Western Blots.Analyze Sanger sequencing data with SnapGene Viewer and microscopy images with ImageJ. Show less
