Adjunct Instructor for Anatomy & Physiology prerequisites for the Junior Dental Hygiene Class.Duties will include but are not limited to: Designing and implementing a 15 week survey of Anatomy & Physiology and lab curriculum.Design lecture content, lab content, assignments, syllabus, prepare and clean up laboratories.

Project: Develop a pure population of ventricular myocytes from adult stem cells (IMR90 iPSc)Research Duties:Design positive and negative selection cassettes to drive ventricular myocyte cardiogenesis.Maintain undifferentiated and differentiated IMR90 iPSC cell linesGenerate transgenic IMR90 iPSC cell lines that will become ventricular cardiomyocytesAcquire and analyze electrophysiology data from cardiac derived IMR90 iPSC cell lines using HEKA Elektronik, IGORPro and AxoGraph. I endorse AxoGraph for all your patch-clamping analysis needs. Reads every possible raw data file common to HEKA and Clampex.

42,000/yr; 50-60hrs/wkProject: Define mechanisms regulating sarcoplasmic reticulum ultrastructure and motility during cardiac Development Research DutiesDesigned, amplified and purified adenovirus constructs for expression in cardiac myocytesSurgically isolated and cultured adult and neonatal rodent cardiac myocytesPreformed electrophysiology assays: Calcium transients and calcium sparks in cardiac myocytes using perforated patch-clamp techniqueOptimized Confocal, Total Internal Reflection Fluorescence (TIRF) microscopy techniques for live cardiac myocyte imagingImmunostaining of intracellular cardiac proteinsParticipated in the development of analytical tools to analyze novel experimental dataWrote and edit manuscripts, prepared figures for manuscripts and presentationsAided in review of journal manuscript articles for principle investigatorSupported colleagues in editing grant applicationsWrote standard operating procedures for laboratory equipmentAdministration DutiesTrained lab personnel on surgical isolation procedures of rodent heartsAided in training of graduate students in experimental techniques and analysisAssisted in the maintenance of the cell culture room to ensure compliance with Biosafety Level 2 (BSL2) standardsCompleted training courses and knowledgeable on topics concerning animal health, wellbeing, use and laboratory surgical protocols for compliance with the Association for Assessment of Laboratory Animal Care (AALAC), and Institutional Animal Care and Use Committee (IACUC)

Part-Time Instructor teaching Anatomy and Physiology I to pre-nursing students.

22,250/yr; 50-60hrs/wkPhD Dissertation: Arrhythmia mutations in the cardiac inward rectifying potassium channel Kir2.1 (KCNJ2): Mechanisms for molecular and cellular phenotypesCreated clinical arrhythmia mutations of Kir2.1 using Site Directed MutagenesisDesigned, amplified and purified adenovirus constructs for expression in cardiac myocytesPreformed electrophysiology assays on Kir2.1 wild-type and arrhythmia mutations in heterologous cells (HEK293, Cos-1), adult and neonatal rodent cardiac myocytes using whole-cell patch-clamp technique to record current-voltage relationships or current-clamp technique to record action potentialsImmunostaining and western blotting of Kir2.1 in HEK293 cellsWrote manuscripts, prepared presentations for scientific meetings, aided in writing grant applicationsAdministration DutiesManaged the maintenance and defrosting schedule of the labs eight -20C freezersCompleted and took regular courses on animal health, wellbeing and use to remain in compliance with Association for Assessment of Laboratory Animal Care (AALAC)Monitored undergraduate student research projects

Salary; 10-20hrs/wkProject: Antibiotic resistance rhizosphere soil bacteria in unfarmed land of the Mount Union College Nature Center.* Isolated and purified antibiotic resistant bacteria strains from unfarmed rhizosphere soil layers* Identified bacteria genus and species*Isolated and purified plasmid DNA from antibiotic resistant bacteria

Assist professor in laboratory clean upTutor students in laboratory topicsAssist professor in teaching students laboratory topics

3,000/mo; 40hrs/wk Project: The structure, function and differences of inactivation mechanisms between the Kv4.3 and shaker voltage gated potassium channelsDesigned and engineered genetic mutants of the Kv4.3 voltage-gated potassium channel to resemble inactivation domain of shaker voltage-gated potassium channelRecorded inactivation currents from Kv4.3 mutant channels via two-electrode whole-cell voltage patch-clamp in Xenopus laevis oocytes