Amanda Wise Email & Phone Number

Lenexa, Kansas, United States

Leads meetings with sponsor and operations teams to facilitate project completion.Deliver the project within agreed upon timeline and budget, communicating with internal teams and the sponsor when out of scope work may be required.Provide notification of milestone completions for billing to financePrimary client communication focal point for project team for bioanalytical studiesWork as primary point of contact between PRA Clinic and PRA Bioanalytical Lab when the study is a "One PRA" contractTrain other PM’s and support staff within the group Part of the Innovative teams working to update internal LIMS and LES systemsAttend Bid Defenses as required and communicate with potential sponsors and current sponsors about future pipelines or assets.

Lenexa, Ks

Study Director and/or Principle Investigator over Large Molecule and Flow Cytometry in the Bioanalytical Lab for studies ranging from pre-clinical to late phase clinical. I work with several dozen Pharmaceutical and Biotechnology companies to plan, design and execute projects in both pre-clinical and clinical phases. The client therapeutics we are currently evaluating range target Alzheimer's, schizophrenia, specific cancer therapeutics, immune diseases (SLE and crohns disease), Gastrointestinal (Celiac, inflammatory diseases) and more.PRA’s North American Bioanalytical Laboratory in Lenexa provides small and large molecule analytical capabilities for pre-clinical and Phase I-IV trials and is fully harmonized with the Assen laboratory, therefore adhering to the same stringent guidelines.PRA’s Bioanalytical Laboratories: Service Offerings in Europe & North America• Mass Spectrometry;• Ultra / High Performance Liquid Chromatography;• Ligand Binding Assays;• Biochemistry (stimulation assays, enzymatic activity);• PK analysis of Small Molecules;• PK/PD/Biomarkers analysis of Large Molecules;• Immunogenicity (ADA) analysis (from Q4 – 2014 also neutralizing antibody measurement);• Flow Cytometry Unit;• Pharmaceutical Analysis;• Biohazard Laboratory with a Bio-Safety Level II (ML-II), qualified to handle samples potentially infected with, amongst others, HEP B, HIV and mycobacterium tuberculosis. Analysis of samples in the Biohazard Laboratory will be performed only when samples are Level II, or on specific Sponsor’s request. If samples are to be analyzed in the Biohazard Laboratory, it will be specified in this proposal.

General Chemistry with lab, College Chem I and College Chem II (with labs)-Prepare Lesson Plans-Design lectures and innovative labs-Maintenance of lab supplies and equipment-Grading rubric and application

Hematology And Transplant Research Group

-Administrative duties: -Maintain grants of all 3 PIs -Purchase and stock all inventory-Mentor and train all junior scientists (graduate students, medical students, research assistants)-Design experiments with multi-step processing-Collaborate with other labs and institutes for matters of overlapping expertise-Writing/maintaining animal protocols-Data analysis and statistics-Cell Culture in 2D and 3D co-culture platforms-Harvesting primary cells from patient samples (peripheral blood or bone marrow)Western blots-Plasmid growth and purification-Transfection-Flow Cytometry -Animal work-Hyperbaric Oxygen studies-Clinical Studies-ELISA

Lenexa

I worked with the Analytical and Raw Material teams testing both finished products and raw materials with a range of analytical chemistry tools. I performed moisture testing (Karl Fischer, LOD, ROI), purity assay titrations, species origination (ouchterlony) testing, HPLC amino identification, and bench chemistry to identify the product and impurities. Responsibilities- Site-wide coordination of the qualification/requalification testing program of raw materials for our formulations. - Meet Lead times for various client needs with raw material release- Work with client requests to identify issues with product in a timely fashion- Method Development for new raw material products (mostly for purity assays) - Member of the Raw Material Review Board - Bringing issues with material purity, biotoxicity, and other non-standard materials for discussion with an interdepartmental team- Collaboration with supply chain, purchasing project managers, R&D, QC, and QA plus other smaller groups- Method Transfer

Lipid Biochemistry, under the mentorship of Alex Moise, PhD.Skills acquired:Insect Cell CultureAnimal Work:Tagging, Tail clipping and Genotyping, Maintaning the databaseProduction of LentivirusPlaque Assay Viral TitrationBaculovirus construction and infectionReal Time PCRHPLC - Agilent SystemFPLC - for protein purificationEnzymatic/Phospholipase Activity AssaysImmunoblottingIndependent experimental designAdipogenesis Differentiation, Oil Red-O stainingMentoring Undergraduate StudentsPresenting Data in a formal setting

under the direction of Faris Farassati, PhD, PharmD.Skills acquired:Cell CultureshRNA transduction Plasmid replicationWestern BlottingImmunoprecipitationAffinity assaysCell invasion assaysCancer stem cell sortingInvoicing/Purchases for the entire labSome grant writing experienceAnimal Care and Use Protocol writing experienceMentoring/Training the fellows and new technicians entering the labCoordinated the setup of the lab when we moved in 2009Creation of several data templates used for Data analysis.

Bio-assay developmentSkills acquired: Cell culture maintenance (melanoma) Cell Based assay Client driven protocols

Scientist I, In Vitro potency development under the direction of Thomas Hickey, PhDSkills acquired:Research and Development of ELISA AssaysOptimization of ELISA potencyOptimized ELISA testing under multiple antigen matricesData AnalyisisGLP data collection and processing (compliant with USDA and EU regulations)Handled direct contact with QC/QA on validated productsCell culture for antibody productionBiotinylation of antibodiesHigh proficiencies using relative potency programs, (eg RelPot, TableCurve, Sigma Plot)

Entomology Lab under the direction of Dr. Matthew O'Neal, PhDSample collection and analysis of research materials relating to the increase of soybean aphids after pesticide application

Lab intern under the direction of Marie Koenigsfeld, PhDSkills Acquired: Protein purification using columns Bacterial colonies: growth and manipulation Cell culture maintenance PCR Bradford Assays Making reagents, running DNA and protein gels, making buffers

Master’S Degree, Pharmacology And Toxicology

Pharmacology, Toxicology

Pharmacology, Toxicology And Experimental Therapeutics

Bachelors Of Science, Genetics

Bachelor Of Science - Bs, Genetics