Amanda Miles Email & Phone Number

California, United States

Watertown, Massachusetts, United States

Watertown, Massachusetts, United States

• Collaboratively design and execute NHP validation studies of top performing AAV variants in the eye as the ocular expert on the team• Independently employ techniques including cloning (Gibson, Gateway), ddPCR, SDS-PAGE and Western blots, in vitro assays (HEK293 and retinal organoids) and histology techniques and analysis to test and evaluate AAVs performance• Write and synthesize study reports and findings to present to the company • Simultaneously manage multiple CROs, to optimize outcomes and meet the study goals • Build in-house capabilities of histology and imaging through equipment and material procurement, and protocol development • Work collaboratively across multi disciplinary teams to bridge my biology expertise with computational biologists and company goals

Completed Thesis: 'The Role of dmbx1 and pcdh15 in Zebrafish Retinal Development and Disease'See Publications & Awards below

• Supervised 9 other TAs and collaborated with the 2 teaching instructors on the organization and preparation of the learning material, tutorials, assignments and final tests of a 3rd year advanced genetics course

• Was the primary lab teaching lead for first year 'Introductory Cell and Molecular Biology' (BIO130) labs (2014-2016) where I trained first year students (25+ classes) in basic laboratory techniques related to DNA extraction, PCR and cloning, and graded their assignments. • Was the primary lab teaching lead in second year 'Animal Physiology' labs (2014-2016) where I trained and guided second year biology students in gathering physiological recordings, result acquisition, presenting data in written assignments and writing sections of a research report (i.e. introduction, results or discussion sections) and graded their assignments. • Was the primary tutorial teaching lead in third year 'Eukaryotic Gene Expression' course (2017-2020) where I individually led tutorial-based learning groups of 20+ students each year focused on critically analyzing research papers and designing novel research proposals with my guidance and feedback. I also participated in the design of the assignments and tests with a group of 9 TAs and 2 professors and graded numerous student assignments.• I was nominated for the TA teaching excellence award in 2015

• Redesigned the lab component of the 1st year introductory molecular biology course into an online format used by 1000+ students by re-writing all lab modules and instructionsl; focused concepts on experimental design and methodology to enhance the virtual lab learning experience while integrating scientifically relevant topics (i.e. covid)

Department Of Biochemistry- Dr. Trevor Moraes Lab

• Assisted graduate students with solution and reagent preparation; maintained stocks of consumable products (i.e. pipette tips) and cleanliness of lab to ensure an efficient work environment

Doctor Of Philosophy - Phd, Cell/Cellular And Molecular Biology

• My thesis research focused on the study of retinal development and disease with a focus on photoreceptor cells. • I used zebrafish.

Bachelor Of Science - Bs, Cell/Cellular And Molecular Biology, Honours And High Distinction.

Activities and Societies: Member of the Cell & Systems Biology Student Union. Was IT Director.• cGPA: 3.97/4.0 • Cell and Molecular.

High School Diploma