Ambrine Chacko Email & Phone Number

Richmond, Va, Us

United States

• Cell isolation and general lab maintenance work by following standard operating procedure (SOP) and quality assurance/quality Control (QA/QC) system under general and technical supervision.• Performs quality control procedures to ensure accuracy of clinical data.• Validates test results through correlation with test parameters and QC metrics.• Performs preventative maintenance and troubleshooting on instruments• Adheres to the laboratory’s quality control policies, document all quality control activities, instrument and procedural calibrations and maintenance performed.• Documents all corrective actions taken when test systems deviate from the laboratory’s established performance specifications.• Understands safety procedures relating to a laboratory to include handling of infectious biological agents as well as potentially hazardous chemicals; appropriate hazardous clean up procedures; universal precautions and infection control procedures.• Performs other duties as necessary. Assist in new product/process development, to optimize and validate assay performance and improve laboratory processes. • Works as part of a multi-disciplinary scientific team to implement experimental and manufacturing plans and achieve project goals.• Maintain Occupational Health & Safety, Regulatory and IACUC Compliance as needed.• Perform verification of calibration and preventive maintenance of equipment and keep the equipment in general working order within the laboratory.• Responsible for establishing, executing, and maintaining a quality assurance program that ensures quality control procedures and practices are being used.• Monitor the suppliers to determine if Service Level Agreements and performance levels are being adhered to.• Develop, modify, apply, and maintain standards for quality operating methods and procedures used across the program.• Conduct and/or participate in formal and informal reviews at pre-determined points throughout the development life cycle.

Richmond, Virginia Area

As a part of the research team, oversaw developing strategies, brainstorming and worked together as a team to bring together diverse skillsets and expertise.• Maintain mouse and human cell lines.• Brain tissue lysis techniques using a centrifuge and sonicator.• Protein concentrations using BCA assay.• RNA quantification using nanodrop.• Western Blots using gel electrophoresis.• Cryo sectioning techniques.• Immunohistochemistry and Immunocytochemistry.• Ability to use fluorescence microscope and light microscope.• Sample processing techniques for Mass spectroscopy. Using various filters to separate proteins and peptide fractions.• Various techniques used for the extraction of exosomes using separation techniques.

Mangalore Area, India

QC Chemistry & Biochemistry Worked in Strides Arcolab (India) posted in Analytical research section with R&D supporting team.Primary duties included, but not limited to:• Manage daily activities of multiple areas of QC testing by graduate level scientists involved in release and stability testing. • Review results in conformance with respective specifications and documents. • Conduct and oversee analytical method development, validation and technology transfer to support Quality Control (QC) testing. • Provide scientific and technical expertise in instrument troubleshooting, laboratory/ manufacturing investigations and manufacturing related problem solving. • Conduct API release testing using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR). • Support testing for investigations and identification of by-products and impurities using HPLC, LC-MS, GC-MS and NMR. • Perform release testing of pharmaceutical In-Process components, Finished Products, and Stability samples using Dissolution system, Fourier transform infrared spectroscopy(FT-IR), Ultraviolet visible spectroscopy(UV-Vis) and HPLC.

400701, Navi Mumbai, Maharashtra, India

Ms Biochemistry

Bs Biochemistry