Amin Espah Borujeni Email & Phone Number

Summary:- chemical engineer turned synthetic biologist, with a deep understanding of biological processes (from small molecules to genome-wide interactions) with >15 years of experience in both dry and wet labs. - extensive experience in discovering new mechanisms inside cells, developing biophysical models, and translating the biophysical knowledge into user-friendly software for use in academia and industry. - expert in RNA biology and biophysical modelling of mRNA translation regulation. - expert in precision design of complex genetic systems with keen eye for detail. - efficient leader, skilled at coordinating diverse team members. - superior ability to develop coherent presentations.Synthetic biology:- construction of genome-integrated genetic circuits in bacteria- development of probabilistic (Bayesian) data-driven models to predict host-circuit relationshipBiophysical modeling and discoveries:- discovered several energetic interactions between ribosome and structured 5’ UTRs in bacteria - discovered Ribosome Drafting mechanism for thermodynamically non-equilibrium mRNA translationSoftware development:Ribosome Binding Site (RBS) Calculator (v2.0) – a predictive thermodynamic model of translation initiationRiboswitch Calculator (v1.0) – a predictive thermodynamic model of riboswitch regulation Small RNA Calculator (v1.0) – a predictive thermodynamic model of small RNA regulation Comprehensive Genetic Circuit Analyzer (v1.0) – a toolbox for characterization and debugging of genetic circuits using RNA sequencing and ribosome profiling Notable skills:Synthetic Biology: bacterial genome engineering, complex genetic circuit design and construction Metabolic engineering: flux balance analysis (FBA)Molecular Biology: RT-qPCR, RNA sequencing, ribosome profiling, flow cytometry, cell-free systemsRNA Chemistry: UV melt analysis, ITC, TCSPC, DSC Computer: Python, Matlab, NGS analysis, Aspen Hysys, ChemStation, PyMol, DecayFit, Adobe IllustratorAdvanced Mathematics: Markov model, linear programming, perturbation analysis, asymptotic expansion

Listed skills include Chemical Engineering, Thermodynamics, Matlab, Modeling, and 23 others.