Amy Steadman Email and Phone Number
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Senior researcher and project lead specializing in infectious disease diagnostic assay development and validation. Eight years of experience establishing molecular diagnostics evaluations in global disease-endemic settings. Special interests include novel assay development for respiratory disease, with a focus on non-sputum testing for tuberculosis.LEADERSHIP AND LABORATORY EXPERTISEProject Leadership: Leadership of large scientific teams; technical direction, planning, and execution of early stage research and commercial assay development; management of external partnerships, including academic collaborators, NGOs, commercial partners, field study partners; in-country study design and oversight; presentation of strategy and results to key stakeholders.Bioinformatics: Development and execution of bioinformatics pipelines for nucleic acid sequence analysis, thermodynamic modeling of molecular interactions, genomic interrogation, and assay design. Molecular techniques: Multiplex PCR, digital PCR, and isothermal amplification design, optimization, and execution; nucleic acid isolation technique design, development, and execution; DNA and protein characterization techniques, including electrophoresis, restriction enzyme digest, ligation, transformation, western blot, Fragment Analyzer; nucleic acid sequencing techniques including NGS, Sanger sequencing (ABI 3730xL); FISH.Screening: High-throughput ELISA, ELISA development (including PK and receptor occupancy), multiparameter flow cytometry (LSR II, HyperCyt).Cell Biology: Generation of expression vectors, stable and transient transfection and culture, isolation and culture of primary cell lines.Automation: Operation and programming of Biomek FX, operation and troubleshooting of Agilent Biocel (high-throughput transfection, harvest, protein purification, etc.), use of HyperCyt (high-throughput flow cytometry), ABI 3730xL DNA analyzer, Qpix2.Phage Display: Selection of phage bound to target antigen immobilized on magnetic beads, polystyrene tubes or plates, and/or mammalian cell surfaces. Utilization of high-throughput screening and sequencing to isolate relevant antibodies.Software: Geneious, Vector NTI, Prism GraphPad, FlowJo, Diva, ForeCyt, SoftMax, Excel
Gates Foundation
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Gates Foundation
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Senior Research ScientistGh Labs Dec 2021 - PresentSeattle, Washington, UsAs a project lead and Senior Research Scientist at Global Health Labs (formerly the Intellectual Ventures Laboratory and Global Good Fund), I am in charge of strategy and leadership of a multi-year, multi-million dollar novel medical diagnostic research and development project. I manage a large number of external partnerships with universities, governmental organizations, NGOs, and commercial entities. I am responsible for study design, training, and execution at four international sites. I also provide technical consultation to multiple IVD companies. -
Project Leadership • Bioinformatics • StrategyGh Labs Aug 2020 - Dec 2021Seattle, Washington, Us
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Research Scientist And Project LeadIntellectual Ventures Laboratory And The Global Good Fund Aug 2017 - Aug 2020
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Associate Scientist, BiotechnologyIntellectual Ventures Laboratory And The Global Good Fund Jan 2017 - Aug 2017
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Staff Scientist, BiotechnologyIntellectual Ventures Laboratory And The Global Good Fund Nov 2015 - Dec 2016
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Senior Research AssociateSengine Precision Medicine Aug 2015 - Nov 2015
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Research AssociateNovo Nordisk Nov 2010 - Nov 2014Bagsværd, DkExecuted and optimized the phage display process, contributing to tangible results as measured by generation of high-quality antibodies to defined target proteins. Designed and operated high-throughput in-vitro assays to validate phage display-derived antibodies and support multiple drug discovery efforts. -- Isolated highly-specific antibodies to many targets in various stages of pipeline, including late-stage clinical trials, often under pressure of short timelines.-- Cloned target-specific antibodies into mammalian vector system, then transfected mammalian cells to produce IgG or F(ab). -- Performed high-throughput sequencing and analysis to identify unique clones. -- Designed and operated high-throughput FACS and ELISA assays to validate resulting antibodies.-- Synthesized and cloned constructs for generation of cell lines for cell-based phage selection.-- Developed and ran pK and receptor occupancy ELISAs, allowing project team to reach definitive answer for path forward with lead antibody.-- Assisted in design and managed database for antibody storage and analysis in collaboration with global scientific computing team. -
ResearchUniversity Of Washington May 2009 - Oct 2010Seattle, Wa, UsDeveloped, optimized, and executed various assays characterizing immunomodulatory biopharmaceutical candidates for B cell activation and tolerance induction. Maintained accurate record keeping and data validation, including data analysis and generation of publication-ready text. -- Operated and optimized cell phenotyping, binding, proliferation, and activation assays using multiparameter flow cytometry and ELISA.-- Cultured human and murine cell lines, both adherent and in suspension, including isolation and culture of murine primary immune cells.-- Performed affinity chromatography, TOPO cloning, PCR, DNA and protein gel electrophoresis, and western blotting. -
Medical Executive AssistantOffice Of Daniel S Frank, Md Apr 2006 - Jan 2010Provided administrative, management, and clinical duties, including personnel training, design and implementation of office protocol, and assisting physician in patient care.-- Trained personnel, including HIPAA compliance and electronic data security training.-- Established and maintained employee manuals and inventory ordering and tracking systems. -- Edited and designed healthcare newsletter featuring articles on current health and well-being topics. -- Audited medical charts to ensure tracking and follow-up of patient health, and performed extremely accurate medical transcription.-- Assisted physical with suturing, biopsies, culture collection, preparation, staining and analysis.
Amy Steadman Skills
Amy Steadman Education Details
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University Of WashingtonBiology -
University Of Southern CaliforniaMusic Industry
Frequently Asked Questions about Amy Steadman
What company does Amy Steadman work for?
Amy Steadman works for Gates Foundation
What is Amy Steadman's role at the current company?
Amy Steadman's current role is Project Leadership • Senior Scientist at GH Labs.
What is Amy Steadman's email address?
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What is Amy Steadman's direct phone number?
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What schools did Amy Steadman attend?
Amy Steadman attended University Of Washington, University Of Southern California.
What are some of Amy Steadman's interests?
Amy Steadman has interest in Animal Welfare, Health.
What skills is Amy Steadman known for?
Amy Steadman has skills like Elisa, Molecular Biology, Pcr, Drug Discovery, Antibodies, Immunology, Western Blotting, Biotechnology, Cell, Flow Cytometry, Phage Display, Cell Culture.
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