Managing a large group of 17-25 people, ensuring that all appropriate paperwork and inductions are completed by new starts.Managing expenditures on multiple grants; prioritising spending in alignment with grant assignments, end dates, and balances.Maintaining lab equipment and communal supplies along with specialised orders.Writing protocols for existing and new procedures.New lab skills:*Extracting primary cells from human explants and maintaining them as a cell culture until they are confluent enough to be frozen and stored. *Immunofluorescent characterisation of these cells to see what ranger were smooth muscle cells, fibroblasts and endothelial cells.*Performing RNA-scope using automated Bond robots and imaging results with *Fluorescent immunocytochemistry staining of zebrafish heart tissue.*Expanding primary cardiomyocyte cell cultures and extracting extracellular vesicles from them in normal oxygen and hypoxic conditions.*Extracellular vesicle (EV) collection from hypoxic and normoxic condition media.*Fractionation of EVs through size exclusion chromatography*Extracellular vesicle characterisation by NanoSight flow cytometry

As the research assistant in a molecular virology lab studying Ovine Pulmonary Adenocarcinoma (OPA), my role is to provide lab-based support to the clinical trial of OPA infection. The overall aim of the study is to develop a model of pulmonary lung cancer that can be used in human diagnostics, alongside the development of a vaccine for the Jaagsiekte respiratory virus (JSRV) that causes OPA in sheep and goats. I was responsible for planing and executing my own work to deliver results that carry the project forward. I analysed, interpreted and discussed the scientific data my work produces with my PI on a regular basis and suggested next steps.Techniques and and skills used in this role include: manual RNA extraction from exhale breath condensate, lung fluid and nasal swabs; RT-qPCR using ABI7500 and QuantStudio5 thermocyclers; Post mortem removal of lungs and lymph nodes from sheep and preparing tissue for histology and DNA extraction; Purification of plasmids from E.coli; DNA extraction from lung tissue; Handling, manipulating, updating and maintaining large data sets; Working at ISO9001 GLP standards.

Working as Research Assistant for the Jonhe's group, I provide technical support in the diagnosis of Johne's disease in ovine and bovine herds in Scotland.My day to day work involves culturing M. avium subspecies paratuberculosis (MAP) on plates, slopes and in suspension, maintaining these cultures and producing growth curves of each species to determining its growth cycle; developing and optimising a phage plaque assay as a diagnostic tool for identifying MAP infection and quantifying MAP culture viability; liaising with farmers to set up surveillance projects on sheep flocks to detect MAP and parasite co-infection in live farms; On-farm work sampling animals and soil for active infections; Working at ISO9001 GLP standards and applying QA requirements to diagnostic testing.I was heavily involved in a gut loop infection study, managing the cultures used to inoculate the gut loops, determining their optimal infection growth stage; producing the inoculum; assessing its viability on the day of infection; and preparing the gut tissue, post mortem, for analysis, such as histology, bacterial recovery and RNA sequencing. I was the deputy Point of Contact for the CL2 lab in which several groups are working on a variety of projects and was jointly responsible for maintenance of the lab and its equipment, and ensuring it operates within the Code of Conduct and H&S guidelines. This also included reviewing and updating the SOPs and Risk Assessments for all MAP work.

The purpose of the brand new virology lab was to test for positive SARS-CoV-2 samples in populations of Care Home staff, prison staff, hospital staff, clinical trials and pre-op or ward admitted patients by RT-qPCR during the Covid-19 pandemic.I was an integral participant in the setup and running of the lab, being one of the first 6 people to open the lab use to my CL3 Covid-19 experience. I was heavily involved in calibrating new equipment, testing and validating new procedures, performing analyser comparisons for UKAS accreditation, and training new staff. I was actively involved in management communications regarding creating and improving SOPs. My role involved supervising and problem solving in different areas of the lab, requiring in-depth knowledge of each part of the process to troubleshoot errors on the large scale automation liquid handling Hamilton robots, extraction robots and thermocyclers, and keep the lab functioning at a high throughput capacity while maintaining the highest standards of quality control procedures. I had to keep up to date with any changes that occurred while I was working elsewhere in the process.As a key member of the training team, I was responsible for training new staff members. As each new group of healthcare assistants started in the lab, I was responsible for their education and training in safely handling and deactivating live virus samples in Microbiological Safety Cabinets (MSC).I was responsible for assessing competencies of other lab members and updating the competency records to accurately reflect abilities of each individual.

As part of an NHS subcontracted lab, I worked under tight deadlines during the Covid‑19 pandemic performing virology diagnostics with a 24 hour turn around time. This was done in a Containment Level 3 (CL3) laboratory using Class II Microbiology Safety Cabinets (MBSCs)an strict adherence to Standard Operating Procedures (SOPs), and included full training in CL3 pathogen handling and waste disposal. All work was carried out at ISO 9001:2015 standards of quality assurance and under Good Laboratory Practice.The process involved working with live virus samples and highly toxic chemicals, and required an understanding of the COSHH and safety procedures associated with these chemicals as well as what to do in the case of an accidental chemical or biological spill.A high level of team work was required to coordinate each stage of the operation. Each job linked directly to the next stage of the process and required a high level of good communication to ensure it proceeded smoothly. I often took over work from other team members to allow for breaks or handed work over, meaning that all work had to be done methodically to be easily followed by someone else. At all times, it was important that I was aware of what was going on in the lab around me and how my work fitted in to the overall process.

As the technician for the Plant Cell Wall group, I was part of developing research into the structure of the cell wall and the manipulation of its components through novel proteins and enzymes. My skills in this role have included radioactive labelling of proteins, paper chromatography, TLC running and staining, tissue culture maintenance of plant and algal cultures, enzyme digests and separation by size exclusion columns. On top of this, I was also responsible for managing the lab; maintaining equipment and chemical stocks, liaising with other departments such as stores, the workshop, other labs and IT when required, monitoring radioactivity usage, health and safety and taking care of PhD students within the lab.

My responsibilities include preparing equipment, reagents and materials for practical classes; maintaining, repairing, calibrating and portable appliance testing of equipment; completing risk assessments for designated practical courses and liaising with academic staff regarding their course requirements.

Assisting Amazon.co.uk customers with any Kindle related queries via phone, e-mail and video link. Topics include order issues, payments methods, content not downloaded, applications not responding, Wi-Fi connectivity issues, device malfunctions and general how-to questions regarding any of the Kindle devices. I was involved in the launch of Mayday, which is an innovative form of customer service that allows the customer to see the tech adviser as they talk and gives control of the device to the tech adviser so that they can efficiently talk the customer through solving the problem and even do it themselves if need be.As well as Kindle specific support, I also provided technical support for all of Amazon.co.uk digital services, such as Amazon Music, Prime Instant Video and Cloud Drive.

Assisting customers with any mortgage queries via phone. Customer queries would include general information about their mortgage, editing their details such as correspondence address and direct debit date or amount and taking payments towards the mortgage.

Duties included taking calls and emails from customers as part of Amazon’scustomer service network. Was responsible for resolving queries from customersin relation to their account, orders, deliveries and pre-orders.

As part of a 14-man Organisation with a turnover in excess of 1 million dollars, personally generated $17,800 over a 13-week period as a dealer based in Colorado, USA, working an average of over 80 hrs a week in direct sales and marketing. Finished as the Top Female First-Year Dealer within the organisation.