Ana-Maria Cujba Email & Phone Number

London, Gb

Cambridgeshire, England, United Kingdom

Computational biologist position, focused on single-cell data analysis for multiple projects, spanning from airway organoid models for COVID-19 infection, and atlas building of human immune cells and vasculature across tissues under Human Cell Atlas. Highly collaborative position, with contributions to other internal or external projects.

London, United Kingdom

Modelling Monogenic Diabetes using human induced pluripotent stem cell-derived pancreatic organoids - Coding using R, Unix, HTML, basic Python, use of High-Performance Computer Cluster - Exome and transcriptome sequencing analysis, basic analysis in single cell RNA sequencing - iPSC and organoid cell culture, differentiation to pancreatic progenitors/beta-cells - Genome editing using CRISPR-CAS9, confocal and high-content imaging (Operetta), molecular biology• Invited oral talks:3rd London Stem Cell Network - 1 December 2020King's Crick PhD symposium - 2 February 2020• Conference poster presentations: 6th International Stem Cell Symposium, Cambridge University - 21st September 20182nd Stem Cell Network Symposium, Francis Crick Institute - 30th May 2019NYSCF Conference, New York, USA - 22nd October 2019

London, England, United Kingdom

Rotation projects: - Dr Tamir Rashid (stem cell-based therapies for end-stage liver diseases) - Prof Giovanna Lombardi (use of regulatory T cell adoptive transfer for autoimmune diseases)- Dr Rocio Sancho (regulation of pancreatic transcription factors for beta-cell development) - Lectures, workshops and assessments focused on stem cell research- Reports and presentations on rotations projects and a main PhD project proposal

Aberdeen, United Kingdom

Year V: Honours project on cell-based therapy approaches for Type 1 Diabetes - Prof Kevin Docherty. Worked on the characterization of mesenchymal stem cells as in intermediary step of reprogramming of human pancreatic exocrine tissue into beta-cells)- mammalian cell culture, immunoflorescence, immunocytometry, qPCR analysis, phenotypical characterization and biology of MSCs.Year IV: Industrial Placement, with project focused on the development of proteolysis targeting chimeras (PROTACs) drug candidates against cancer, R&D Biological Sciences, GlaxoSmithKline, Stevenage, UK Year III-I:• Courses in Biochemistry, Immunology, Molecular Biology, Cell Biology, Physiology, Genetics• Project on African Sleeping Sickness as part of the Aberdeen 2014 team

Stevenage, United Kingdom

Worked on development of Proteolysis Targeting Chimera (PROTAC) drug candidates. Acquired skills in insect cell culture, Baculovirus generation, protein purification (affinity, ion-exchange and size-exclusion chromatography), MALDI- TOF, LC-MS mass spectrometry, peptide mass fingerprinting and fluorescence polarization assays. Delivered oral and poster presentations for group and departmental meetings, teaching secondary school students mass spectrometry and insect cell culture.

Aberdeen, United Kingdom

Member of the 2014 Aberdeen iGEM team - used synthetic biology approaches to design Escherichia detect antibodies in the early stages of infection with the Trypanosoma brucei parasite, which causes African Sleeping Sickness. The goal of the project was to replace heavy and bulky equipment needed for the current diagnosis with a diagnostic backpack, based on pre-made E. coli cultures. Delivered a poster and PowerPoint Presentation at the Giant Jamboree at MIT in October 2014, where we competed against 245 international teams. As part of the project, we also promoted synthetic biology and our project among experts in the field and the general public, by taking part in a Synthetic Biology Conference in Edinburgh, writing blog-post, series of radio interviews, talks and making a website.

Aberdeen, United Kingdom

Careers Subject Representative for the School of Medical Sciences. I collaborated with Careers Advisers and employers to deliver career prospects among fellow students. Organized the first Undergraduate Biomedical Careers Conference on, with more than 150 students and a diversity of top speakers from academia and industry attending the event.

Aberdeen, Scotland

Member of the ‘First Aid’ Society (AUSA Red Cross Group) and secretary of the group, operated as a messenger between staff, committee and Society members. Organized the Students Fare during Fresher’s Week, sent informative e-mails regarding forthcoming events on a weekly basis and organized social events for the society.

Aberdeen, United Kingdom

Establishing communication between students and members of staff, provide feedback from students to course coordinators and organising study groups.

Aberdeen, Scotland

• Performing Western Blotting and gel electrophoresis.• Working with the Opsys MR™ Microplate Reader computer program.

Brasov, Romania

• Practicing the Gram staining protocol supervised by staff members.• Working with immunological (Immulite 1000 immunoassay system) and biochemical apparatus, observing incubation protocols and blood testing procedures.• Understanding analysis tests and diagnosis results.

Master In Science (Msci), Biochemistry (Immunology), 1St Class Honours Degree

Level III courses: Molecular Biology of the Cell, Frontiers of Molecular Medical Sciences, Placement and Career Skills, Genetics.

A Level / Scottish Highers, Natural Sciences

Diploma of Baccalaureate in: Organic chemistry (9.80), Mathematics (9.35), Romanian language (8.35) *Romanian marking scale (from 1.