Ancy Thomas, Ph.D Email & Phone Number

Oakland, Ca, Us

Berkeley, Ca, Us

Evanston, Il, Us

• Generated human and mouse CAR-T cells and engineered TCR transduced T-cells in multiple immunotherapy projects with leading roles in experiment design and execution, data analysis and interpretation, documentation and presentation.• Performed multicolor flow cytometry-based experiments in conventional and spectral FACS platforms for the characterization of immune cells and tumor cells from patient and mouse samples.• Developed and optimized in vitro and ex vivo bioassays to study T cell proliferation, activation and tumor-immune cell interactions using ELISA, Immunofluorescence, IncuCyte and Isoplexis platforms.• Designed and executed extensive in vivo studies in syngeneic, spontaneous and PDX tumor models; performed routine injections (sc, ip, iv) in mice, monitored tumors, collected blood and tissue samples and recorded data for the pre-clinical validation of therapeutic T cells.• Collaborated with multiple teams of scientists and clinicians in the solid tumor immunotherapy program; prepared reports, communicated research updates periodically, and presented findings to other internal and external groups.• Mentored graduate students and actively participated in training and onboarding of new team members; functioned as a safety manager of the lab monitoring and ensuring compliance of good GLP and OSHA practices, IACUC animal regulations etc.

Chicago, Il, Us

• Investigated the epigenetics and chromatin regulation in T-cell Acute Lymphoblastic Leukemia utilizing advanced techniques in molecular biology and immunology• Explored the role of adenovirus proteins in the transcription biology and molecular signaling of prostate cancer• Examined the functions of DNA modifying enzymes such as histone acetyltransferase in the growth and replication of adenovirus• Optimized methods/protocols (CRISPR, shRNA, immunodetection) to study the functions of DNA binding oncoproteins and tumor suppressors and their cross-talk in the cellular microenvironment

Kerala, In

• Investigated the molecular pathways and mechanism of regulation of innate immune system of invertebrates using shRNA gene silencing in primary cell culture• Instructed undergraduate students and evaluated their lab component, participated in teaching and training of course material, including molecular cloning and recombinant protein production

Chennai, Tamil Nadu, In

• Standardized systems for the development of recombinant protein based antiviral vaccine; performed molecular cloning in bacterial (E.coli) and yeast (Pichia Pastoris) systems and optimized production and purification of protein vaccine candidates using chromatography.• Performed extensive gene and protein sequence analysis and protein modelling using bioinformatics tools for the optimization of recombinant protein production • Executed in vivo experiments in animal models to test the efficacy of vaccine candidates and conducted comprehensive gene expression analysis using RT-PCR• Developed polyclonal antibodies in mice and optimized assays/processes for the purification, quantification and characterization of antibodies and other recombinant proteins such as lysozyme• Experienced in using relevant online data resources and tools for scientific research; prepared and reviewed scientific publications, reports, and protocols. Participated in writing grant applications, prepared progress reports, and presented the summary before grant agencies• Actively participated in supervision, training and evaluation of team members and undergraduate students; instructed courses in immunology and evaluated

Post Baccalaureate Program, Business Communication And Management

Entrepreneurship Acceleration Program

Ph.D, Molecular Biology And Immunology

Master’S Degree