Andrea Castaldi Email & Phone Number

Villejuif, Fr

Parigi, Ile-De-France, Francia

ED227 « Sciences de la nature et de l'Homme : évolution et écologie »Processus d'adhésion des diatomées : vers l’élucidation du mécanisme moléculaireThe core of this research is to describe as precisely as possible the mechanisms involved by fouling actors in the adhesion process by combining two approaches, one transcriptomic and the other metabolomic. We also plan to describe the molecular mechanisms of adaptation of these organisms by varying the coatings proposed for our… Show more ED227 « Sciences de la nature et de l'Homme : évolution et écologie »Processus d'adhésion des diatomées : vers l’élucidation du mécanisme moléculaireThe core of this research is to describe as precisely as possible the mechanisms involved by fouling actors in the adhesion process by combining two approaches, one transcriptomic and the other metabolomic. We also plan to describe the molecular mechanisms of adaptation of these organisms by varying the coatings proposed for our biological models. Our first results on Phaeodactylum tricornutum showed different metabolomic profiles between adherent (glass and poystyrene) and non-adherent (stock and planktonic) diatoms models. O2PLS models classified adherent and non adherent cell cultures through analyses of NMR and HPLC data according to predictive 1. Stock cell cultures were discriminated from other cell cultures according to predictive 2. Around 15 discriminants spectral regions have been pointed out in analyses of HPLC and NMR data, expecting promising metabolites of interest. Further investigations including LC/MS-MS, molecular networking and multi-block analyses will be performed with two other diatoms Amphora coffaeformis and Cylindrotheca closterium cultivated on three surfaces (glass, polystyrene and polydimethylsiloxane) that should provide news insights into molecular mechanisms and lead to promising perspectives in elucidation of antifouling targets. By comparing their responses to those obtained on a reference surface, we will be able to highlight the proposed adaptations according to the supposed difficulty of colonizing other coatings. In addition to the contribution of knowledge on the biology of these organisms, this work should also provide guidance for the development of innovative antifouling coatings which would find their applications at the level of the hulls of vessels, fishing and aquaculture equipment, materials for the production of marine renewable energy, for example. Show less

Parigi, Ile-De-France, Francia

Deciphering mechanism of diatoms adhesion.Biofouling is an inherent phenomenon to all submerged structures: ship hulls, aquaculture equipment, marine energy installations, that enforces to the implementation of costly preventive and curative measures. Societal, environmental and regulatory pressures lead to a strong restriction of the use of toxic compounds present in antifouling formulations. A promising strategy to fight fouling is based on the surface properties of materials and/or… Show more Deciphering mechanism of diatoms adhesion.Biofouling is an inherent phenomenon to all submerged structures: ship hulls, aquaculture equipment, marine energy installations, that enforces to the implementation of costly preventive and curative measures. Societal, environmental and regulatory pressures lead to a strong restriction of the use of toxic compounds present in antifouling formulations. A promising strategy to fight fouling is based on the surface properties of materials and/or coatings (low energy, roughness), which consists in reducing the forces of interaction between organisms and surfaces. Another one could be to better understand the biofilm installation upon surfaces.Benthic diatoms are, with bacteria, the early and major players in surface colonization, forming a tenacious and resilient biofilm. However, so far, diatoms have not been studied extensively, in particular, the knowledge of the process involved in their adhesion remains under-explored. The few available publications underline the importance of better understanding the role of various factors that can be notably decisive in the adhesion of diatoms: topography of the surface, conditioning by exoproducts (exopolysaccharides, communication molecules). Show less

Parigi, Ile-De-France, Francia

Exploring the process of diatoms adhesion using NMR metabolomics.In order to explore diatoms adhesion strategies, we have investigated at a molecular level how the diatom Phaeodactylum tricornutum modifies its metabolism for performing its adhesion according to the encountered substrate. Therefore, we applied proton NMR metabolomics to diatoms adherent to glass and polystyrene and two planktonic non-adherent diatom cultures.

Parigi, Ile-De-France, Francia

Chemical investigation of the marine sponge holobiont Rhabdastrella globostellataIn the framework of the Erasmus+ traineeship project from October 2019 to Avril 2020, I was hosted at the Muséum National d’Histoire Naturelle (MNHN) of Paris at the laboratory “Molécules de Communication et Adaptation des Micro-organismes (MCAM)”, a mixed research Unit under the dual supervision of the CNRS (INEE) and MNHN. This research work was supervised by Dr. Marie-Lise Bourguet-Kondracki, Research… Show more Chemical investigation of the marine sponge holobiont Rhabdastrella globostellataIn the framework of the Erasmus+ traineeship project from October 2019 to Avril 2020, I was hosted at the Muséum National d’Histoire Naturelle (MNHN) of Paris at the laboratory “Molécules de Communication et Adaptation des Micro-organismes (MCAM)”, a mixed research Unit under the dual supervision of the CNRS (INEE) and MNHN. This research work was supervised by Dr. Marie-Lise Bourguet-Kondracki, Research Director, CNRS in the team “Biochimie des Intercations Microbiennes (BIM)”. The work was developed in the frame of a collaboration with the Marine Biochemistry Institute (IMBC) from Vietnam Academy of Science and Technology (VAST) in Hanoi (Vietnam). I had the opportunity to work on the research project focused on chemical investigation of marine sponge holobionts. Particularly, I was involved in the isolation, purification and structure elucidation of compounds from the Streptomyces sp. G222 isolate, a bacterial strain associated with the marine sponge Rhabdastrella globostellata, collected off the Ha Long Bay in Vietnam. Show less

Master Of Science - Ms In Medicinal Chemistry And Technology, Chimica E Tecnologia Farmaceutiche, 110/110 With First Class Honours.

The Master of Science in Medicinal Chemistry and Technology in addition to basic preparation for the profession of Pharmacist, develops.

Erasmus+, Pharmacy

UE25 – Pathologies 3 – Clinical Hematology – 5 ECTS UE 25 – Pathologies 3 – Clinical Immunology – 5 ECTS UE 30 – Pathophysiology.

High School Diploma, 100/100

Laurea Triennale, Saxofono Jazz

Exams passed: - Videoscrittura musicale ed editoria musicale informatizzata - Teoria e tecnica dell’armonia - Teoria della musica.