In our GMP laboratory, my colleagues and I do several in-process controls for HOLOCLAR, an ATMP based on ex vivo expanded autologous human corneal epithelial cells containing stem cells, approved for the treatment of Limbal Stem Cell Deficiency, and for other projects in clinical trials (Hologene 5 for example).These assays include immunofluorescence for the identification of corneal epithelial and stem cell markers and microscope analysis to determine the clonogenicity of cultured… Show more In our GMP laboratory, my colleagues and I do several in-process controls for HOLOCLAR, an ATMP based on ex vivo expanded autologous human corneal epithelial cells containing stem cells, approved for the treatment of Limbal Stem Cell Deficiency, and for other projects in clinical trials (Hologene 5 for example).These assays include immunofluorescence for the identification of corneal epithelial and stem cell markers and microscope analysis to determine the clonogenicity of cultured cells.Moreover, we have the routine activities of the laboratory, such as preparing solutions, writing SOPs, recording data in batch records, performing validations and calibrations of new systems and instruments, and more. Show less