Andre Phan Email & Phone Number

Waltham, Ma, Us

• Synthesize polymer particles using established chemistries• Perform analytical characterization of polymeric particles using particle sizing

South San Francisco, California, Us

• Purified IgG1 antibodies from CHO expression using affinity column and SEC column on AKTA avant • Utilized HPLC for expression titer and to determine protein purity • Measured protein concentration using nanodrop or SoloVPE

San Jose, Ca, Us

• Utilized plate reader based enzyme coupled assay of hSIRT1-143 with various peptides to determine effect of small molecule modulators on Michaelis-Menten enzymatic activity• Interpret, summarize, and presented poster at national conferences hosted by ASBMB (2023) and Protein Society (2022)• Utilized protein purification using Ni2+ column and SEC column on AKTA FPLC and characterized protein after using SDS-PAGE• Conduct transformation of hSIRT1-143 plasmid to E.Coli• Perform protein expression of hSIRT1-143 in E.Coli to prepare for protein purification• Measure hSIRT1 concentration using Abs280 and Bradford Assay

Raritan, New Jersey, Us

• Developed influenza polymerase replication FRET assay to support biochemical mechanism of action (MOA) studies• Conducted high throughput screens against hundreds of inhibitors using FRET transcription assay• Utilized FRET and radiometric filter plate assay to determine substrate affinity• Interpret, summarize and present findings of assay development during internal team meetings

• Performed plasmid prep, purification, digest and screening of pGEM• Conducted sequencing analysis on plasmid to discover multiple mutations• Established large scale digest of a purified PCR product to add a second restriction site