Andreia Cepeda, Phd Email & Phone Number

Leiden, Zh, Nl

Amsterdam, Noord-Holland, Nl

• Production of recombinant adeno-associated virus (rAAV) viral vectors for nonclinical gene therapy projects.• Coordination of USP, DSP, and QC activities.• Lead QC.• Data analysis and interpretation, and reporting to stakeholders.• LIMS, GxP, Review of Electronic Lab notebook registries, Preparation of Review Documentation (study designs, summaries, reports), SOPs and Work Instructions (writing, review, update).• Supply chain management, Shipments, and Inventory control.

Göttingen, Niedersachsen, De

• Led scientific projects for high-throughput detection, quantification, and validation of biomarkers (Proteomics / Neuroscience).• Performed experimental design, plans, budgeting, timeline, prioritizing tasks, data analysis, wrote reports and SOPs, reported scientific activities to internal and external team and project leaders and clients, ensured transfer of SOPs, generated dissemination materials, wrote scientific articles.• Supported operations of the in-house Mass Spectrometry core facility.• Guided and supervised technical personnel and students.

Us

Göttingen, Lower Saxony, De

• Explored protein function in the hearing field and contributed to a dual-AAV gene therapy approach that restored hearing in adeafness mouse model. Performed in vitro and in vivo assays in cell and tissue culture and rodents.• Developed biochemical, affinity-based methods, cell-based assays, and established screening platforms for interactome studies in cell lines and tissues of rodent models of disease.• Wrote project proposals, reports, protocols, SOPs.• Participated in national and international collaborations, presented data at scientific meetings.• Laboratory management, stock supply, responsible for molecular biology and biochemistry areas and for chromatography instrumentation, biosafety advocate for BSL1 (culture room, cell bank, reagents).Achievements:• Adapted single-cell immunoassay / microscopy-based assay to inner ear tissue, reducing screening time by 90%.• Discovered new key protein interactions and protein modifications that helped understanding synaptic transmission from inner ear synapses.• Helped develop a dual-AAV gene therapy that restored hearing in a deafness mouse model.

Göttingen, Lower Saxony, De

• Studied synaptic vesicle recycling defects and neurodegeneration in mouse models of disease. Extensive technical input and laboratory effort led to publication of a proposed neuronal cell death pathway in the journal Cell Reports.• Fueled and maintained the entire research pipeline: maintained animal models of neurological and neurodegenerative diseases, harvested organs (macro- and microdissections), established primary neuronal and chromaffin cell cultures, produced lentiviruses, maintained cell culture lines on biosafety levels 1 and 2 (BSL-1 and BSL-2) according to GLP and GMP, adapted and optimized gene delivery into mammalian cells via lipofection and electroporation.• Implemented and initiated bacterial cell expression systems and chromatography-based purification methods in the group; installed new AKTA system, performed maintenance, trained users

Oeiras, Pt

• Manufacturing of recombinant proteins – small scale and upscaling (engineering, production, purification, and high-throughput screening) for two research projects: 1) haem proteins for potential application in bioremediation of contaminated environments or in energy production by microbial fuel cells. 2) antimicrobial peptides for application in the food industry.• Presented data in internal meetings, prepared project documentation (protocols, SOPs, project plans, summaries, reports).• Responsible for laboratory management (inventory, instrument maintenance).

Madrid, Comunidad De Madrid, Es

• Provided and received training in international collaboration project to study the structure and mode of action of a new bacteriocin with potential applications in the food industry.

Lisboa, Lisboa, Pt

• Conducted laboratory work in the fields of biochemistry and redox metabolism, where I initiated a study of the impact of a reactive oxygen species in cell metabolism, gene expression, and cell survival. My findings were published in the scientific journal Yeast.• Collected and analysed data, performed literature search, participated in manuscript and abstract writing for publications and presentations. Presentation of data and scientific articles in internal meetings and conferences.

Postgraduate Specialization, Drug Development Product Management

Doctor Of Philosophy (Ph.D.), Sensory And Motor Neuroscience

Master Of Science (M.Sc.), Biochemistry (Medical Biochemistry Specialization)

Bachelor Of Science (B.Sc.), Biochemistry