Andrew Proffitt Email and Phone Number

- Lead a team supporting Early Discovery efforts in the Rare Disease Unit- Build in vitro disease models for target identification and validation, and for screening assays during drug discovery- Manage external academic collaborations to accelerate technology development and target identification efforts for the Rare Disease unit- Utilize -omics, AI and other Data Science tools in early discovery for novel target identification and to provide support to biological hypotheses

• Manager of the Expression Sciences group that supports the production of reagents through the cloning and generation of DNA, protein expression, purification and QC of biological molecule• Utilize -omics, AI and other computational efforts and implement those tools in early discovery for new target identification and validation

• Support Discovery Research with target identification and validation with internal and external resources• Utilize -omics, AI and other computational efforts and implement those tools in early discovery • Managed the production of reagents through the cloning and generation of DNA, protein expression, purification and QC of biological molecules• Supported preclinical development of antibody therapeutics and ongoing qualification using a diverse set of tools including FACS, protein chemistry, and cell based assays• Responsible for the writing of scientific and technical documents and supported IND submissions

Research Scientist with Kolltan Pharmaceuticals 07/15-10/16 and acquired by Celldex Therapeutics (11/16 to present)• Managed the production of reagents through the cloning and generation of DNA, protein expression, purification and QC of biological molecules• Provided reagents for in vitro and in vivo assays for company-wide groups and sites• Supported preclinical development of antibody therapeutics and ongoing qualification using a diverse set of tools including FACS, protein chemistry, and cell based assays• Responsible for the writing of scientific and technical documents and supported IND submissions

Molecular Biologist, Operations and Manufacturing• Manufactured HLA antigen detection and SSO typing kits• Operated cross functionally between manufacturing, supply chain, QA and QC• Executed tasks in an ISO and GMP certified environment, managed an ERP system, ensured rigorous documentation, and developed their C3d product SOPs• Documented non-conformances with CAPA, published discrepancy reports, and facilitated root-cause laboratory investigations

Research and Development in the Immunology Department• Completed several internal and external service contracts utilizing mammalian and bacterial cell culture; includes cell-based assays, protein production (including subcloning genes of interest), purification and characterization• Independently developed and launched multiple products for the detection of pathogens in multiple formats (ELISA, lateral flow, and PCR based products)• Generated over 60 monoclonal antibodies against 12 different antigens from initial immunizations of mice to final subcloning and thorough characterizations of each clone using hybridoma technology• Involved in antibody production and characterization including affinity measurements, epitope mapping, isotype determination, identifying sandwich pairs, competition ELISAs, and cell-based tests for therapeutic

Research in molecular biology and protein chemistryCloning of proteins in the TNF/TNFR superfamily of proteins using a novel expression system.Stable line construction for the purpose of protein manufacturing in mammalian expression systems.Protein purification and biochemical characterization.Drug developmentScreening hybridoma clones for binding affinity and concentration of antibodies produced.Obtain the sequence of the variable regions of the antibodies to eliminate redundant clones and generate recombinant antibodies.

Biochemical research, natural product isolation and structure determination Used standard enzymological and molecular biological techniques in the cloning, purification, characterization and kinetic analysis of enzymes in the nonmevalonate biosynthetic pathway. Includes aerobic and anaerobic techniques and electron paramagnetic resonance characterizations. Worked on natural product extraction and structural determination from uncharacterized Pacific marine organisms.

Analytical determination of phthalates in marine environments under Harry Pylypiw Ph. D. Worked on methods development for the extraction and purification of phthalates in synthetic marine environments. Includes work with various extraction and chromatographic methods.

Assisted with the organization of their method developments and a wide range of chemical analyses for their proprietary products and private contracts. Managed document organization and storage. Laboratory experience in a GMP and GLP regulated environment.